液相色谱法快速检测染色梅鱼中日落黄、柠檬黄的含量

Rapid detection of sunset yellow and lemon yellow in Collichthys niveatus by liquid chromatography

中文关键词:  日落黄  柠檬黄  梅鱼  液相色谱法

英文关键词:sunset yellow  lemon yellow  Collichthys niveatus  liquid chromatography

中文摘要:

      目的 建立一种快速、低成本检测染色梅鱼中日落黄、柠檬黄的方法。方法 将染色梅鱼剥皮, 鱼皮经70%甲醇氨水提取液提取, 经Eclipse XDB-C18色谱柱分离, 以甲醇和乙酸铵水溶液为流动相, 进行梯度洗脱, 流速为1.0 mL/min, 柱温35 ℃, 利用紫外检测器检测日落黄、柠檬黄的含量。结果 日落黄、柠檬黄在1.25、2.5、12.5、25 mg/kg添加水平的加标回收率为80%~90%, 相对标准偏差小于10%(n=6), 柠檬黄检出限为0.05 mg/kg, 日落黄检出限为0.025 mg/kg。结论 该方法快速、准确、成本低, 适合染色梅鱼中日落黄、柠檬黄的快速检测。

英文摘要:

      Objective To develop a method for determination the sunset yellow and lemon yellow in Collichthys niveatus by liquid chromatography. Methods The dyed Collichthys niveatus was peeled, and the fish skin was extracted with 70% methanol ammonia water extract. The samples were separated with a Eclipse XDB-C18 column at the flow rate of 1.0 mL/min by gradient elution using methanol and ammonium acetate in aqueous solution as the mobile phase, the temperature of column was 35 ℃, and the content of sunset yellow and lemon yellow was detected by ultraviolet detector. Results The standard recoveries of sunset yellow and lemon yellow at 1.25, 2.5, 12.5, 25 mg/kg were 80%~90%, and the relative standard deviations were less than 10%(n=6). The detection limit of lemon yellow was 0.05mg/kg, and the detection limit of sunset yellow was 0.025mg/kg. Conclusion This method is rapid, accurate and low cost, which is suitable for the rapid detection of sunset yellow and lemon yellow in the dyed Collichthys niveatus.