高效液相色谱法测定保健食品中番茄红素的含量

Determination of lycopene in health food by high performance liquid chromatography

中文关键词:  番茄红素  保健食品  高效液相色谱法

英文关键词:lycopene  health food  high performance liquid chromatography

中文摘要:

      目的 建立保健食品中番茄红素高效液相色谱法(high performance liquid chromatography, HPLC)含量测定方法。方法 色谱柱为Grace Apollo C18柱(250 mm×4.6 mm, 5 μm), 以甲醇-乙酸乙酯(95:5, V:V)为流动相等度洗脱, 柱温30 ℃, 流速1 mL/min, 检测波长472 nm。结果 番茄红素在1.01~50.45 μg/mL浓度范围内线性关系良好(r2=0.9997), 高、中、低3个浓度水平下的加样回收率为96.20%~105.60%, 相对标准偏差(relative standard deviation, RSD)值不大于2.50%。采用该方法对市售7个厂家的产品进行测定, 发现不同厂家产品中番茄红素的含量相差很大, 其中3个厂家的含量低于其标示量, 质量问题值得引起关注。结论 本文建立的方法简单、准确, 同时精密度、重复性良好, 是对现有国家标准方法GB/T 22249-2008的有效改进, 可用于番茄红素保健食品的质量控制。

英文摘要:

      Objective To establish a method for determination of lycopene in health food by high performance liquid chromatography (HPLC). Methods Chromatographic separation was performed on Grace Apollo C18 column (250 mm×4.6 mm, 5 μm) at 30 ℃ of column temperature. Methanol-ethyl acetate (95:5, V:V) was adopted as mobile phase for isocratic elution. The flow rate was at 1 mL/min. The detection wavelength was 472 nm. Results Lycopene had a good linear relationship in the concentration range of 1.01~50.45 mg/mL (r2=0.9997). And the recoveries were between 96.20% and 105.60% at 3 different levels of concentration with RSD≤2.50%. This method was used to determine the products of 7 manufacturers in the market, and it was found that the content of lycopene in different manufacturers varied greatly, among which the content of 3 manufacturers was lower than the labeled quantity, and the quality problem was worthy of attention. Conclusion This method is simple, accurate, precise and repeatable, which is an effective improvement on the existing national standard method GB/T 22249-2008 and can be used for quality control of lycopene in health food.