液相色谱法同时测定除草剂中乙草胺、异噁草酮和2,4-滴丁酯的含量

Simultaneous determination of acetochlor, clomazone and 2,4-D butylate in herbicides by liquid chromatography

中文关键词:  除草剂  乙草胺  异噁草酮  2,4-滴丁酯  高效液相色谱法

英文关键词:herbicides  acetochlor  clomazone  2,4-D butylate  liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定除草剂中异噁草酮、乙草胺和2,4-滴丁酯的含量。方法 试样经甲醇溶解, 采用ODS-2反相色谱柱, 以甲醇/乙腈/氨水溶液(42:42:16, V:V:V)为流动相, 对试样中异噁草酮、乙草胺和2,4-滴丁酯进行分离, 流速1.0 mL/min, 柱温40 ℃, 紫外检测器检测波长为235 nm, 外标法定量。结果 在测定浓度范围内, 乙草胺, 异噁草酮和2,4-滴丁酯标准溶液质量浓度与峰面积均呈现出良好的线性关系(r>0.9998), 对同一样品重复测定5次, 变异系数分别为0.33%、0.44%和0.41%, 该方法平均加标回收率为98.6%~102.8%, 变异系数为0.28%~0.45%。结论 该方法简便、快捷、精密度和准确度高、线性关系好, 适用于同时测定除草剂中异噁草酮、乙草胺和2,4-滴丁酯含量。

英文摘要:

      Objective To establish a method for simultaneous determination of clomazone, acetochlor and 2, 4-D butylate in herbicides by liquid chromatography. Methods The samples were dissolved in methanol and separated by ODS-2 reversed phase column with methanol:acetonitrile:ammonia solution (42:42:16, V:V:V) as the mobile phase. The flow rate was 1 mL/min and the column temperature was 40 ℃. The detection wavelength of ultraviolet detector was 235 nm, it was quantitated by external standard method. Results There was a good linear relationship between the concentration and peak area of clomazone, acetochlor and 2,4-D butylate (r>0.9998) in the range of determination, respectively. Repeated determination of the same sample 5 times, the coefficient of variation was 0.33%, 0.44% and 0.41%, respectively. The average recovery rates of the method were 98.6%-102.8%, and the coefficient of variations were 0.28%-0.45%. Conclusions This method displays the advantages of convenience, celerity, high precision and accuracy, and good linear correlations, and it is suitable for the simultaneous determination of clomazone, acetochlor and 2,4-D butylate in herbicide.