超高效液相色谱法同时测定功能性饮品和抗疲劳类保健食品中非法添加的11种化学药物

Simultaneous determination of 11 kinds of illegally added chemical drugs in unctional drinks and anti-fatigue health foods by ultra performance liquid chromatography

中文关键词:  保健食品  非法添加  超高效液相色谱法

英文关键词:health foods  illegally additives  ultra performance liquid chromatography

中文摘要:

      目的 建立超高效液相色谱法(ultra performance liquid chromatography, UPLC)同时测定功能性饮品和抗疲劳类保健食品中非法添加的11种化学药物。方法 采用Waters BEH C18柱(100 mm×2.1 mm, 1.7 μm), 以甲醇-10 mmol/L乙酸铵溶液(冰醋酸调pH至3.5)为流动相进行梯度洗脱, 柱温35 ℃, 流速0.2 mL/min。二极管阵列检测, 外标法定量。结果 11种化合物在浓度为1.0~50 μg/mL范围内线性关系良好(r>0.998)。在10、20、40 μg/mL 3个添加水平的回收率为91.8%~104.6%, 相对标准偏差小于5.0%。结论 该法具有较好的专属性、线性、回收率和精密度, 可用于抗疲劳类保健食品中非法添加的11种化学药物的同时测定。

英文摘要:

      Objective To establish a method for simultaneous determination of 11 kinds of illegally added chemical drugs in unctional drinks and anti-fatigue health foods by ultra performance liquid chromatography (UPLC). Methods The samples were separated with a Waters BEH C18 column (100 mm×2.1 mm, 1.7 μm) at the flow rate of 0.2 mL/min by gradient elution using methanol and 10 mmol/L ammonium acetate solution (adjusted to pH 3.5 with glacial acetic acid) as the mobile phase. The temperature of the column was maintained at 35 ℃, the detection was carried out by photo-diode array and quantitatively analyzed by external standard method. Results The 11 kinds of compounds showed good linear relationships in the concentration range of 1-50 μg/mL (r>0.998). The recoveries were 91.8%-104.6% at the 3 spiked levels of 10, 20 and 40 μg/mL, and the relative standard deviations were less than 5.0%. Conclusion The method has good specificity, linearity, recovery and precision, and can be used for simultaneous determination of 11 kinds of illegally added chemical drugs in anti-fatigue health food.