超高效液相色谱-串联质谱法同时测定水中的呋喃丹、莠去津和微囊藻毒素

Simultaneous determination of carbofuran, atrazine and microcystins-LR in water by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  水  呋喃丹  莠去津  微囊藻毒素-LR  超高效液相色谱-串联质谱法

英文关键词:water  carbofuran  atrazine  microcystin-LR  ultra performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立同时测定水中的呋喃丹、莠去津和微囊藻毒素-LR的超高效液相色谱-串联质谱方法。 方法 样品直接过0.22 μm的水系滤膜, 以0.1%甲酸水溶液(A)和乙腈(B)为流动相经BEH C18柱(100 mm× 2.1 mm, 1.7 μm) 梯度洗脱分离, 串联四极杆质谱在电喷雾正离子(electrospray ionization, ESI+)模式下同时测定呋喃丹、莠去津和微囊藻毒素-LR的含量。结果 呋喃丹、莠去津和微囊藻毒素-LR在0.5~50 ng/mL范围内线性良好, r≥0.999, 平均加标回收率90.2%~99.5%, 精密度为2.1%~4.5%。结论 本方法操作简单、灵敏度高、线性范围宽、结果准确可靠, 可用于水中的呋喃丹、莠去津和微囊藻毒素-LR的同时测定。

英文摘要:

      Objective To establish a method for simultaneous determination of carbofuran, atrazine and microcystins-LR in water by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The samples were directly filtered 0.22 μm water filtration membrane, and then were separated with gradient elution through the BEH C18 column (100 mm×2.1 mm, 1.7 μm) with the mobile phase of 0.1% formic acid aqueous solution (A) and acetonitrile (B). The carbofuran, atrazine and microcystins-LR were determined simultaneously by UPLC-MS/MS in electrospray positive ion (ESI+) mode. Results Carbofuran, atrazine and microcystins-LR had good linear relationships in the range of 0.5-50 ng/mL, and the correlation coefficients were larger than 0.999, the average recoveries were 90.2%-99.5% and the precisions were 2.1%-4.5%. Conclusion The method has the advantages of simple operation, high sensitivity, wide linear range, accurate and reliable results, which can be used for simultaneous determination of carbofuran, atrazine and microcystins-LR in water.