高效液相色谱法测定鱼油软胶囊中维生素D3含量的不确定度评定

Uncertainty evaluation in determination of cholecalciferol in fish oil soft capsule by high performance liquid chromatography

中文关键词:  高效液相色谱法  维生素D3  不确定度  鱼油软胶囊  内标曲线法

英文关键词:high performance liquid chromatography  cholecalciferol  uncertainty  fish oil soft capsule  internal standard curve method

中文摘要:

      目的 评定高效液相色谱法(high performance liquid chromatography, HPLC)测定鱼油软胶囊中维生素D3含量的不确定度。方法 依据国家计量技术规范JJF 1059.1-2012《测量不确定度评定与表示》对维生素D3测定中的不确定度来源进行分析。通过建立数学模型量化不确定度分量, 计算合成不确定度和扩展不确定度。结果 本方法的不确定度主要来源于标准物质的含量、标准曲线的配制和标准曲线的拟合。当样品中维生素D3的含量为2.08 μg/kg, 扩展不确定度为0.20 μg/kg, 维生素D3含量表示为(2.08±0.20) μg/kg (k=2)。 结论 该评定方法可用于高效液相色谱法对鱼油软胶囊中维生素D3的不确定分析。

英文摘要:

      Objective To evaluate the uncertainty of determination of cholecalciferol in fish oil soft capsule by high performance liquid chromatography (HPLC). Methods The source of uncertainty in determination of vitamin D3 was analyzed according to the National Measurement Technical Specification JJF 1059.1-2012 Evaluation and expression of uncertainty in measurement. The combined uncertainty and extended uncertainty were calculated by establishing mathematical models. Results The uncertainty of this method mainly came from the standard substance content, the standard curve formulation and the fitting of the standard curve. When the vitamin D3 content in the sample was 2.08 μg/kg, the expanded uncertainty was 0.20 μg/kg, and the vitamin D3 content was (2.08±0.20) μg/kg (k=2). Conclusion This method can be used for the uncertainty analysis of cholecalciferol in fish oil soft capsules by HPLC.