高效液相色谱法测定不同产地湘莲中荷叶碱、 莲心碱、芦丁的含量

Determination of nuciferine, liensinine, rutin in Hunan lotus seeds from different origins by high performance liquid chromatography

中文关键词:  湘莲  荷叶碱  莲心碱  芦丁  高效液相色谱法

英文关键词:Hunan lotus seeds  nuciferine  liensinine  rutin  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定湘莲中荷叶碱、莲心碱、芦丁的方法。方法 在酸性条件下超声提取3种待测组分。色谱条件如下: C18(250 mm×4.6 mm, 5 μm)色谱柱; 流动相: 甲醇-0.1 mol/L乙酸铵(用乙二胺调节pH值至7.5~8.0)(55:45, V:V); 流速: 1.0 mL/min; 检测波长: 275 nm; 柱温30 ℃; 进样量10 μL。 结果 荷叶碱、莲心碱、芦丁分别在7.636~152.7 μg/mL(r2=0.9999, n=5)、7.543~150.9 μg/mL(r2=0.9995, n=5)、7.543~150.9 μg/mL(r2=0.9998, n=5)范围内线性关系良好, 加标回收率为96.9%~100.5%之间, 相对标准偏差(relative standard deviation, RSD)为1.1%~1.4%。结论 该法同时测定湘莲中荷叶碱、莲心碱、芦丁, 简单快速, 定量准确, 可以为评价不同产地湘莲功效成分提供参考。

英文摘要:

      Objective To establish a method for simultaneous determination of nuciferine, liensinine and rutin in Hunan lotus seeds by high performance liquid chromatography. Methods Totally 3 kinds of components were extracted by ultrasonic under acid condition. The chromatographic conditions were as follows: column was C18 (250 mm×4.6 mm, 5 μm), methanol-0.1 mol/L ammonium acetate (to adjust the pH value to 7.5~8.0 with ethylenediamine) (55:45, V:V) was as mobile phase, flow rate was 1 mL/min, detection wavelength was 275 nm, column temperature was 30 ℃ and sample size was 10 μL. Results The nuciferine, liensinine and rutin showed a good linear range at 7.636~152.7 μg/mL (r2=0.9999, n=5), 7.543~150.9 μg/mL (r2=0.9995, n=5) and 7.543~150.9 μg/mL (r2=0.9998, n=5), respectively. The recoveries were 96.9%~100.5% at the spiked levels with relative standard deviation of 1.1%~1.4%. Conclusion This method is accurate, simple and reliable, and it provides a reference for the evaluation of different origin of lotus ingredients, which can be applied to the simultaneous determination of nuciferine, liensinine and rutin in lotus seeds.