菊苣醇提物对对乙酰氨基酚诱导小鼠肝损伤的保护作用

Protective effect of ethanol extracts of Cichorium intybus L. against acetaminophen-induced liver injury in mice

中文关键词:  菊苣  对乙酰氨基酚  肝损伤  CYP2E1  抗氧化

英文关键词:Cichorium intybus L.  acetaminophen  liver injury  CYP2E1  oxidative stress

中文摘要:

      目的 研究菊苣醇提物对对乙酰氨基酚(acetaminophen, APAP)诱导的小鼠肝损伤的保护作用。方法 将56 只昆明小鼠随机分为7 组: 分别是阴性对照组, APAP 模型组, 水飞蓟宾阳性对照组, 菊苣醇提物单独给药组(200 mg/kg 体重), 菊苣醇提物低、中和高剂量(50、100 和200 mg/kg 体重)与APAP 联用组。各剂量组小鼠灌胃给予对应药物连续7 d 后, 一次性腹腔注射APAP(300 mg/kg 体重)构建肝损伤模型, 24 h 后采集肝组织样品和血清。分别测定肝组织和血清的多种氧化指标以及细胞色素P450 2E1 酶(CYP2E1)的蛋白表达水平。结果 菊苣醇提物能够显著降低血清中谷丙转氨酶(alanine aminotransferase, ALT)和谷草转氨酶(aspartate aminotransferase, AST) 水平。降低肝组织中过氧化氢(hydrogen peroxide, H2O2) 和谷胱甘肽(glutathione, GSH)的含量以及提高抗氧化酶系中过氧化氢酶(catalase, CAT)和总超氧化物歧化酶(totalsuperoxide dismutase, T-SOD)活性; 同时, 体外抗氧化实验亦表明其具有良好的清除自由基的能力。另外, 菊苣醇提物还能够显著抑制CYP2E1 的蛋白表达。结论 菊苣醇提物有助于维持小鼠体内酶防御系统功能, 提高机体清除自由基的能力, 并通过减少CYP2E1 的蛋白表达对APAP 所致小鼠肝损伤具有明显保护作用。

英文摘要:

      Objective To investigate the protective effect of ethanol extracts of Cichorium intybus L. (CI) on acetaminophen (APAP) induced liver injury in mice. Methods Fifty-six Kunming mice were randomly divided into 7 groups: negative control group, APAP model group, silybin positive control group, CI-alone group (200 mg/kg body weight), low, middle and high dose (50, 100, 200 mg/kg body weight) of CI and APAP groups. The liver injury model was established by intraperitoneal injection of APAP (300 mg/kg body weight) after 7 days. After 24 h, the liver tissue and serum were collected. Oxidation indicators and the expression of cytochrome P450 2E1 enzyme(CYP2E1) were detected from liver tissues and serums in seven groups. Results The ethanol extracts of CI could significantly decrease the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum,decrease the content of hepatic hydrogen peroxide (H2O2) and glutathione (GSH), and increase the activities of catalase (CAT) and total superoxide dismutase (T-SOD) in the antioxidant enzymes. The in vitro studies showed that the ethanol extracts of CI had good ability to scavenge free radicals. In addition, the ethanol extracts of CI also significantly inhibited CYP2E1 protein expression. Conclusion The ethanol extracts of CI can help to maintain the function of the anti-oxidative enzyme system in mice and improve the ability to scavenge free radicals in the body,and have obvious protective effect on APAP-induced liver injury in mice through CYP2E1 inhibition.