液相色谱-串联质谱法测定茶叶中的11种农药残留

Determination of 11 pesticide residues in tea by liquid chromatography tandem mass spectrometry

中文关键词:  液相色谱-串联质谱法  茶叶  农药残留  固相萃取

英文关键词:liquid chromatography-tandem mass spectrometry  tea  pesticides  solid phase extraction

中文摘要:

      目的 建立液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)测定茶叶中11种农药残留的分析方法。方法 样品经乙腈提取, 经石墨化炭黑/N-丙基乙二胺复合型固相萃取柱(graphite carbon black/primary secondary amine, GCB/PSA)净化, 用液相色谱-串联质谱法测定。结果 11种农药浓度在0.005~0.20 μg/mL范围内线性良好, 线性相关系数均大于0.997, 在0.01、0.05 和0.10 mg/kg的添加水平下, 回收率为66.0%~100.0%, 相对标准偏差为2.2%~10.0%。结论 本方法简便、快速、准确, 适用于茶叶样品中11种农药残留量的检测。

英文摘要:

      Objective To establish a method for determination of 11 pesticide residues in tea by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods The samples were extracted with acetonitrile, purified by graphite carbon black/primary secondary amine composite type solid phase extraction column (GCB/PSA) and determined by LC-MS/MS. Results Eleven kinds of pesticides had good linear relationships in the range of 0.005~0.20 μg/mL, and the correlation coefficients were above 0.997. The recoveries at 3 spiked levels (0.01, 0.05 and 0.10 mg/kg) were 66.0%~100.0%, with the relative standard deviations between 2.2%~10.0%. Conclusion The established method is simple, rapid, accurate and suitable for the simultaneous determination of 11pesticides in tea.