甘薯蛋白对DMH/DSS诱导的肥胖小鼠炎症相关结肠癌的抑制作用

Suppression of DMH/DSS induced inflammation associated colorectal cancer by sweet potato protein in obese mice

中文关键词:  结直肠癌  甘薯蛋白  化学预防  生物活性成分  小鼠

英文关键词:colorectal cancer  sweet potato protein  chemoprevention  bioactive constituents  mice

中文摘要:

      目的 采用1,2-二甲肼(1,2-dimethylhydrazine, DMH)/右旋葡聚糖苷钠(dextran sodium sulfate, DSS)诱导雄性肥胖ICR小鼠建立炎症相关结肠癌模型, 观察甘薯蛋白(sweet potatoprotein, SPP)的抑制作用及机制。方法 在腹腔注射15 mg/kg的DMH, 1周后给予小鼠1周的2%DSS饮水, 间隔1周后再次给予1周DSS饮水, 循环3次。在给予DMH后第3 d开始灌胃给予不同剂量SPP(0.02、0.2及1 g/kg?bw)至21周。结果 各SPP干预组肿瘤数及恶性腺瘤率较DMH/DSS模型组均降低, SPP干预组癌组织中β-catenin, IGF-1及血管内皮生长因子(vascular endothelial growth factor, VEGF)蛋白表达显著降低。结论 SPP对化学致癌剂/致炎剂诱导的炎性相关性结直肠癌具有显著抑制作用, 其机制与SPP下调癌组织中β-catenin, IGF-1及VEGF蛋白表达有关。

英文摘要:

      Objective To investigate the inhibitory effect of sweet potato protein (SPP) on chemically induced colorectal carcinoma and explore its possible mechanism by using the 1,2-dimethylhydrazine/dextran dextran sodium (DMH/DSS)-induced inflammation-associated colorectal carcinomain obese male mice. Methods Male obese ICR mice were given intraperitoneal injection of 15 mg/kg?bw of DMH. After 1 week, they were given 3 cycles of 2%DSS in drinking water for 1 week and then switched to normal drinking water for 1 week to induce inflammation-associated colorectal carcinoma. SPP was administered intragastrically at 0.02, 0.2 and 1 g/kg?bw until the 21th week. Results The number of tumors and the rate of malignant adenocarcinoma in each SPP intervention group were lower than those in DMH/DSS model group. The expression of β-catenin, insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in tumor tissues were significantly suppressed by SPP. Conclusion SPP significantly inhibits chemical-induced inflammation-associated colorectal cancer, which may be related to the suppression of the expression of β-catenin, IGF-1 and VEGF in inflammation-associated colorectal cancer tissues induced by DMH/DSS.