免疫亲和层析结合高效液相色谱法检测氟喹诺酮类药物

Determination of fluoroquinolones by immunoaffinity chromatography with high performance liquid chromatography

中文关键词:  免疫亲和色谱  氟喹诺酮类  高效液相色谱法

英文关键词:immunoaffinity chromatography  fluoroquinolones  high performance liquid chromatography

中文摘要:

      目的 建立检测氟喹诺酮类药物的免疫亲和层析样品前处理方法结合高效液相色谱法的分析方法。方法 通过将抗恩诺沙星单抗与溴化氰活化的琼脂糖4B偶联作为免疫吸附剂来提取胎牛血清中的5种氟喹诺酮类药物, 并用高效液相色谱仪配备荧光检测器检测。结果 在本研究中, 诺氟沙星、恩诺沙星、环丙沙星、沙拉沙星和达氟沙星在胎牛血清中的萃取回收率分别为82.97%、70.50%、70.16%、31.83%和6.37%, 检出限均为0.5 ng/mL。结论 建立的氟喹诺酮类免疫亲和层析色谱胶具有高的吸附容量、选择性、萃取效率和稳定性。免疫亲和色谱法是一种可以从复杂基质样品中萃取多种氟喹诺酮抗生素的可行方法。

英文摘要:

      Objective To establish a method for determination of fluoroquinolones by immunoaffinity chromatography with high performance liquid chromatography (HPLC). Methods The anti-enrofloxacin monoclonal antibody was covalently cross-linked to CNBr-Sephorase 4B as an immunosorbent agent which was used for the extraction of 5 kinds of fluroquinolones including norfloxacin, enrofloxacin, ciprofloxacin, sarafloxacin and danofloxacin from bovine serum. A liquid chromatography equipped with a fluorescence detector was used for the detection of the extracted fluroquinolones. Results The recovery values of norfloxacin, enrofloxacin, ciprofloxacin, sarafloxacin and danofloxacin in bovine serum were 82.97%、70.50%、70.16%、31.83% and 6.37%, respectively. The limits of quantitation of fluroquinolones were all 0.5 ng/mL. Conclusion The established fluoroquinolone immunoaffinity chromatography displays high binding capacity, selectivity, extraction efficiency and stability. The immunoaffinity chromatography is a feasible method for the extraction of multiple fluoroquinolone antibiotics from complex matrix samples.