QuEChERS-气相色谱-串联质谱法测定香菇中29种农药残留

Determination of 29 kinds of pesticides in Lentinus edodes by QuEChERS-gas chromatography-tandem mass spectrometry

中文关键词:  香菇  农药残留  QuEChERS  气相色谱-串联质谱法

英文关键词:Lentinus edodes  pesticide residues  QuEChERS  gas chromatography-tandem mass spectrometry

中文摘要:

      目的 建立一种检测鲜香菇中毒死蜱等29种农药的QuEChERS-气相色谱-三重四极杆串联质谱仪(gas chromatography–tandem mass spectrometry, GC-MS/MS)的方法。方法 鲜香菇样品经前处理后, C18和N-丙基乙二胺(primary secondary amine, PSA)粉末分散固相萃取净化, 经过DB-5MS毛细管柱分离后, 通过多反应监测(multiple reaction monitoring, MRM)方式测定, 使用内标法定量。结果 29种农药在一定的浓度范围内线性关系良好, 相关系数R2均大于0.99。3个水平的添加回收实验结果显示, 29种农药的平均回收率在90.7%~122.3%之间, 相对标准偏差均小于14.9%。结论 本研究建立了一种QuEChERS-GC-MS/MS方法同时测定鲜香菇中29种农药, 结果快速、准确, 适用于香菇样品的农药残留检测工作。

英文摘要:

      Objective To establish an analytical method for simultaneous determination of multi-residual pesticides in Lentinus edodes by gas chromatography-tandem mass spectrometry (GC-MS/MS) coupled with QuEChERS. Methods After pretreatment, the Lentinus edodes samples were purified by a mixture of sorbents (primary secondary amine and C18). After DB-5MS capillary column separation, the module of multiple reaction monitoring was applied for determination. Internal standard method was used for quantitative analysis. Results The 29 kinds of pesticides had good linear relationships in their linear ranges with correlation coefficients R2>0.99. The recoveries of 29 kinds of pesticides were 90.7%~122.3% spiked at 3 levels and the relative standard deviations were below 14.9%. Conclusion The QuEChERS-GC-MS/MS method is sensitive and accurate, which can be used as routine analysis method in multi-residual pesticides monitoring of Lentinus edodes.