高产γ-氨基丁酸植物乳杆菌的微波诱变育种

Selection of high-yield γ-aminobotyric acid-producing Lactobacillus plantarum by microwave mutagenesis

中文关键词:  微波诱变  γ-氨基丁酸  植物乳杆菌

英文关键词:microwave mutagenesis  γ-aminobotyric acid  Lactobacillus plantarum

中文摘要:

      目的 利用微波辐照对植物乳酸杆菌进行诱变育种, 筛选高产γ-氨基丁酸的正突变菌株。方法 以TYG为发酵培养基, 37.0 ℃培养48 h后, 测定微波诱变后的植物乳杆菌产γ-氨基丁酸的量。结果 诱变后突变菌株W462S5的γ-氨基丁酸的产量为9.18 g/L, 相比于未诱变前的产量(4.64 g/L), 提高了97.84%。对正突变菌株W462S5进行8次传代培养发酵, 测得γ-氨基丁酸的产量较为稳定, 表明W462S5是一株遗传性状稳定的正突变菌株。结论 微波诱变菌株不仅有操作简单、设备常见、实验条件易于控制等优点, 且选育出的菌株具有培养周期短、易于分离纯化、遗传性状稳定等优势。将此方法应用于发酵γ-氨基丁酸生产中, 具有一定的研究意义。

英文摘要:

      Objective To screen a positive mutant strain with high-yield γ-aminobotyric acid by mutagenesis of Lactobacillus plantarum using microwave irradiation. Methods Using TYG as fermentation medium, L. plantarum was fermented at 37.0 ℃ for 48 h, then the yield of γ-aminobotyric acid of L. plantarum was determined after microwave mutagenesis. Results According to the method, compared to the pre mutagenic yield of 4.64 g/L, the content of γ-aminobotyric acid by positive mutant strain of W462S5 was 9.18 g/L, which increased by 97.84%. After 8 generations of subculture fermentation of W462S5, the content of γ-aminobotyric acid was relatively stable. The results showed that W462S5 was an excellent genetic stable mutant strain. Conclusion The microwave mutation strains have the advantages of simple operation, common equipments and easy control of the experimental conditions, and the selected strain has the advantages of short culture period, easy separation and purification, and stable genetic characteristics and so on. The application of this method in the production of γ-aminobotyric acid has certain research significance.