胶体金免疫层析法快速检测烟草中吡虫啉残留

Rapid detection of imidacloprid residues in tobacco leaf by colloidal gold immunochromatographic assay

中文关键词:  吡虫啉  烟草  胶体金免疫层析法  快速检测

英文关键词:imidacloprid  tobacco leaf  gold immunochromatography assay  rapid detection

中文摘要:

      目的 建立胶体金免疫层析法快速检测烟草中残留的吡虫啉。方法 用柠檬酸三钠还原法制备胶体金, 并用它标记吡虫啉单克隆抗体, 建立并优化胶体金免疫层析法。结果 本方法对烟草中吡虫啉的检测限为5 μg/mL, 重复性较高, 有假阳性情况出现, 但假阳性率较低, 与烟草中其他常用药物没有交叉反应, 特异性良好, 且与气相色谱检测法所得的结果差异较小。结论 该方法快速、高效、可靠, 可满足现场快速检测烟草中吡虫啉残留的需要, 实现生物技术在烟草流通、消费领域的方便、快速检测。

英文摘要:

      Objective To establish a rapid method for determination of imidacloprid residues in tobacco leaf by colloidal gold immunochromatographic assay. Methods Colloidal gold was prepared by sodium citrate reduction method, and it was marked imidacloprid monoclonal antibody. Then the method of colloidal gold immunochromatographic assay was built and optimized. Results The results showed that the visual detection limit of imidacloprid was 5 μg/mL, and the reproducibility was high. The false positive condition was found, but the false positive rate was low. There was hardly any cross reaction with other commonly used compounds in tobacco. The results of gold immunochromatographic assay were well consistent with those of gas chromatography method. Conclusion The established gold immunochromatographic assay is rapid, efficient and reliable, and can meet the need of on-site rapid detection of imidacloprid residues in tobacco. Therefore it achieves biotechnology convenient and rapid detection in tobacco circulation and consumption field.