3种沙门氏菌检测方法能力验证

Capability verification of three detection methods of Salmonella

中文关键词:  沙门氏菌  PCR  能力验证

英文关键词:Salmonella  PCR  proficiency test

中文摘要:

      目的 比较国标法、VETIK和荧光定量PCR法3种沙门氏菌检测方法的检测效果。方法 10份盲样经GB4789.4-2010《食品安全国家标准 食品微生物学检验 沙门氏菌检验》、实时荧光定量PCR方法和VIDAS仪器检测, 生化部分用VITEK2 compact执行。结果 国标法在10个盲样中检出3种血清型沙门氏菌, 分别为5号样品检出阿贡纳沙门氏菌、7号样品检出蒙得维的亚沙门氏菌和肯塔基沙门氏菌。VETIK和荧光定量PCR法检出5号和7号呈沙门氏菌阳性, 其他为阴性。结论 VIDAS和实时荧光PCR检测方法快速、可靠、灵敏度高, 可作为传统检测方法有效补充。

英文摘要:

      Objective To compare the detection effects of Salmonella by the national standard method, VETIK and the fluorescence quantitative PCR method. Methods Ten blind samples were detected by GB4789.4-2010 National food safety standard Food microbiological examination: Salmonella, real-time quantitative PCR and VIDAS instrument, and biochemical part was executed by VITEK2 compact. Results Using the national standard, three serotypes of Salmonella serotypes were detected in 10 blind samples. Salmonella agona was detected in sample 5, Salmonella Montevi and Salmonella kentucky were detected in sample 7. VETIK and fluorescence quantitative PCR detection results exhibited that sample 5 and sample 7 were Salmonella positive, the others were negative. Conclusion VIDAS and real-time fluorescence quantitative PCR methods are rapid, reliable and sensitive, and can be used as an effective complement to traditional detection methods.