龟甲粉对小鼠免疫功能的作用研究

Effects of tortoise shell powder of Chinemys reevesii (Gray) on immune function in mice

中文关键词:  龟甲粉  小鼠  免疫作用

英文关键词:tortoise shell powder  mice  immune function

中文摘要:

      目的 研究龟甲粉对昆明种小鼠免疫功能的作用。方法 利用40只昆明种小鼠进行龟甲粉抗体生成细胞测定、对昆明种小鼠HC50的影响、碳廓清能力以及鸡红细胞吞噬率及吞噬指数的影响实验。实验设4组, 灌胃的龟甲超微细粉分别为生理盐水组与不同剂量组, 连续灌胃30 d后测定各项指标。结果 0.3 g/(kg?bw)及0.45 g/(kg?bw)组龟甲超粉可以显著促进小鼠B细胞分泌抗体的能力(P<0.05), 0.45 g/(kg?bw)组龟甲粉能显著增加小鼠半数溶血值(HC50)(P<0.01), 0.15 g/(kg?bw)及0.45 g/(kg?bw)组龟甲粉可以显著提高小鼠单核-巨噬细胞碳廓清能力及小鼠腹腔巨噬细胞鸡红细胞吞噬率及吞噬指数(P<0.01)。结论 龟甲粉可以增强小鼠体液免疫功能和单核-巨噬细胞功能的作用。

英文摘要:

      Objective To evaluate the regulation of tortoise shell powder of Chinemys reevesii (Gray) on immune function in mice. Methods Forty mice were used to conduct antibody-producing cell of spleen, total hemolytic complement assay (HC50 assay), clearance of carbon particles, and chicken red blood cell phagocytic rate and phagocytic index assay. The 40 mice were randomly divided into 4 groups (n=10): 0, 0.15, 0.3 and 0.45 g/(kg?bw) superfine tortoise shell powder of C. reevesii (Gray) were orally administered once daily for 30 days. Results In comparison with the control group, 0.3 g/(kg?bw) and 0.45 g/(kg?bw) superfine tortoise shell powder of C. reevesii (Gray) could significantly increase the ability of mice B cells secreting antibodies (P<0.05), 0.45 g/(kg?bw) superfine tortoise shell powder of C. reevesii (Gray) could significantly increase HC50 value (P<0.01), 0.15 g/(kg?bw) and 0.45 g/(kg?bw) superfine tortoise shell powder of C. reevesii (Gray) could significantly increase the ability of carbon particles clearance by mice macrophages and the phagocytic rate and phagocytic index of chicken red blood cells in mouse peritoneal macrophages (P<0.01). Conclusion The tortoise shell powder of C. reevesii (Gray) can enhance the functions of humoral immune and monocyte macrophage in mice.