全自动石墨消解-电感耦合等离子体质谱测定鱼、虾等生物样品中铜、镉、锌、铬4种重金属元素

Determination of Cu, Cd, Cr and Zn in fish and shrimp by automated graphite digestion combined with inductively coupled plasma mass

中文关键词:  全自动石墨消解  电感耦合等离子体质谱  鱼  虾  重金属

英文关键词:automated graphite digestion  inductively coupled plasma mass spectrometry  fish  prawn  heavy metals

中文摘要:

      目的 建立一种简便、快速的鱼、虾等生物样品中重金属元素的分析方法。方法 以10.0 μg/L的Rh作为内标, 采用硝酸-过氧化氢-高氯酸体系, 用全自动石墨消解仪消解鱼、虾等生物样品, 建立石墨消解-电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)同时测定样品中铜、镉、锌、铬4种金属元素的方法。结果 各元素的检出限在0.001~0.200 μg/L之间, 相对标准偏差在0~2.30%之间, 加标回收率为在91.4%~113.4%之间。对大虾标准品(GSB-28)进行分析的测定值与标准值吻合良好。结论 本研究所建立的方法简单、快速, 灵敏度高, 重现性好, 是同时分析大批生物样品中多种金属元素的可靠、高效方法。

英文摘要:

      Objective To develop an easy, fast, and accurate method for the simultaneous determination of Cu, Cd, Cr and Zn in biological samples like fish and shrimp. Methods Using 10.0 μg/L Rh as internal standard element, nitric acid, hydrogen dioxide and perchloric acid as digestion acids,fish, shrimp and other biological samples were digested by automatic graphite digestion instrument, and the method for the simultaneous determination of 4 metal elements of copper, cadmium, zinc and chromium by graphite digestioncombined with inductively coupled plasma mass spectrometry (ICP-MS) was established. Results The detection limits for each element were in range of 0.001~0.200 μg/L, the relative standard deviations (RSDs) ranged from 0 to 2.3%, and the recoveries were 91.4%~113.4%. The determined values of prawn (GSB-28)were in good agreement with the certified values. Conclusion The developed method is simple, rapid, sensitive and has good reproducibility, which is a reliable and efficient method for simultaneous determination of Cu, Cd, Cr and Zn in large quantities of biological samples.