超声波辅助酶法提取香菇柄中总黄酮的工艺优化

Optimization of the process for the extraction of total flavonoids in stem of Lentinus edodes by ultrasonic assisted enzymatic method

中文关键词:  香菇柄  黄酮类化合物  超声波辅助酶法  响应面分析法

英文关键词:stem of Lentinus edodes  flavonoids compounds  ultrasonic assisted enzymatic method  response surface analysis method

中文摘要:

      目的 对采用超声波辅助酶法提取香菇柄中黄酮类化合物的工艺进行优化, 为进一步开发香菇柄资源提供依据。方法 以总黄酮得率为指标, 通过单因素试验研究乙醇体积分数、超声时间、超声功率及酶用量对黄酮类化合物得率的影响, 利用响应面分析法对影响黄酮类化合物得率的上述4个因素进行优化。结果 最佳提取工艺参数为: 乙醇体积分数75%、超声时间20 min、超声功率280 W、酶用量0.012 mg。在此条件下, 通过3次验证实验, 测得黄酮类化合物的得率为1.673 mg/g, 实际值与理论值基本吻合。结论 采用响应面分析法优化超声波辅助酶法提取香菇柄中的黄酮类化合物的工艺具有可行性。

英文摘要:

      Objective To optimize the extraction process of flavonoids compounds from the stems of Lentinus edodes by ultrasonic assisted enzymatic method, so as to provide a basis for the further development of stems of Lentinus edodes resources. Methods With the yield of total flavonoids as the index, the effects of ethanol concentration, ultrasonic time, ultrasonic power and enzyme dosage on the yield of flavonoids compounds were studied through the single-factor test, and the above 4 factors were optimized by response surface analysis method. Results The optimum parameters in extraction process were as follows: the ethanol concentration was 75%, the ultrasonic time was 20 min, the ultrasonic power was 280 W, and the enzyme dosage was 0.012 mg. Under these conditions, the yield of flavonoids was 1.673 mg/g through 3 confirmatory experiments, and the actual value was in good agreement with the theoretical value. Conclusion It is feasible to use response surface analysis method to optimize the extraction process of flavonoids compounds from the stems of Lentinus edodes by ultrasonic assisted enzymatic method.