电感耦合等离子体-质谱法与氢化物原子荧光法 测定小麦中总硒含量的方法比较

Comparison of quantification methods of selenium in wheat with hydride generation-atomic fluorescence spectrometry and inductively coupled plasma-mass spectrometry

中文关键词:  氢化物原子荧光法  电感耦合等离子体-质谱法  小麦  硒

英文关键词:hydride generation-atomic fluorescence spectrometry  inductively coupled plasma-mass spectrometry  wheat  selenium

中文摘要:

      目的 比较分析电感耦合等离子体-质谱法与氢化物原子荧光法测定小麦中硒含量。方法 用氢化物原子荧光法(hydride generation-atomic fluorescence spectrometry, HG-AFS)和电感耦合等离子体-质谱法(inductively coupled plasma-mass spectrometry, ICP-MS)两种方法测定小麦样品中硒的含量, 从检出限(LOD)、精密度(RSD)及回收率等方面进行方法学考察, 其中前者采用微波消解后需继续用电热板加热消解, 并加入盐酸将六价硒还原为四价硒的前处理方法, 后者采用仅采用微波消解的前处理方法。结果 HG-AFS法的LOD为0.016 μg/L, RSD为1.00%, 回收率为94.0%~110.5%; ICP-MS法的LOD为0.028 μg/L, RSD为4.06%, 回收率为89.0%~116.6%。结论 两种方法检测结果基本一致, 均可以准确、灵敏地检测小麦样品中硒的含量。

英文摘要:

      Objective To compare hydride generation-atomic fluorescence spectrometry (HG-AFS) and inductively coupled plasma-mass spectrometry (ICP-MS) for the determination of selenium in wheat. Methods The content of selenium in wheat was determined by HG-AFS and ICP-MS under the optimal conditions. The limit of detection (LOD), the precision (RSD), and the recovery were investigated and compared. Electric heating plate digestion was carried out after microwave digestion by adding HCl for the reduction of Se6+ to Se4+ in the pretreatment processing of HG-AFS, while only microwave digestion was performed in ICP-MS. Results The LODs of HG-AFS and ICP-MS were 0.016 μg/L and 0.028 μg/L, RSDs were 1.00% and 4.06%, the recovery ranges were 94.0%~110.5% and 89.0%~116.6%, respectively. Conclusion As the similar performances including good repeatability and high sensitivity, HG-AFS and ICP-MS can be used as the routine methods for the determination of selenium in wheat.