QuEChERS-高效液相色谱-串联质谱法同时测定 果蔬中26种植物生长调节剂残留

Simultaneous determination of 26 kinds of plant growth regulator residues in fruits and vegetable by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

中文关键词:  植物生长调节剂  QuEChERS  高效液相色谱-串联质谱法

英文关键词:plant growth regulators  QuEChERS  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立高效液相色谱-串联质谱法同时测定果蔬中26种植物生长调节剂残留量的检测方法。方法 样品经QuEChERS法预处理, 用含1%甲酸的乙腈溶液提取, 无水硫酸镁和十八烷基硅烷(C18)粉末净化, 以Agilent Poroshell 120 EC-C18 (3.0 mm×100 mm, 2.7 μm)为分析色谱柱, 甲醇和5 mmol/L乙酸铵-0.1%甲酸缓冲液作为流动相进行梯度洗脱分离, 采用电喷雾多反应监测模式, 基质匹配标准溶液外标法定量。结果 26种植物生长调节剂线性良好, 线性相关系数均>0.990, 该方法的检出限在1~10 μg/kg之间, 以豆芽和苹果为代表性基质进行3个水平加标回收实验, 回收率在80.81%~118.4%之间, 相对标准偏差(RSD)为0.9%~14.8%。结论 该方法灵敏度高, 定性、定量准确, 简便高效, 可适用于多种水果和蔬菜中26种植物生长调节剂残留的同时检测要求。

英文摘要:

      Objective To establish an analytical method for the simultaneous detection of 26 kinds of plant growth regulator residues in fruits and vegetable by using QuEChERS-high performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS). Methods Samples were pretreatment by QuEchERS and extracted with acetonitrile containing 1% acetic acid, the extracts were cleaned up with sorbents (MgSO4 and C18), and carried out on Agilent Poroshell 120 EC-C18 (3.0 mm×100 mm, 2.7 μm) column with 5 mmol/L ammonium acetate solution (containing 0.1% formic acid) and methanol as the mobile phases. The samples were detected by tandem quadrupole mass spectrometry with multiple reaction monitoring (MRM) and quantified by matrix-matched external standard method. Results The limits of detection of the method were in ranged of 1~10 μg/kg. The calibration curves showed a good linearity in each concentration with r>0.990, and the recoveries of 26 kinds of plant growth regulators spiked at 3 levels in bean sprout and apple were 80.81%~118.4%, with the relative standard deviations (RSDs) ranged from 0.9% to 14.8%. Conclusion The method is simple, convenient, sensitive and accurate, and suitable for the simultaneous detection of 26 kinds of plant growth regulator residues in fruits and vegetables. KEY WORDS: plant growth regulators; QuEChERS; high performance liquid chromatography-tandem mass spectrometry