高效液相色谱法测定婴幼儿配方米粉和乳粉中 烟酸和烟酰胺含量

Determination of nicotinic acid and nicotinamide in infant formula milk powder and rice powder by high performance liquid chromatography

中文关键词:  烟酸  烟酰胺  高效液相色谱法  米粉  奶粉

英文关键词:nicotinic acid  nicotinamide  high performance liquid chromatography  rice powder  milk powder

中文摘要:

      目的 建立高效液相色谱法测定婴幼儿配方米粉和乳粉中烟酸和烟酰胺的提取分析方法。方法 婴幼儿配方米粉和乳粉以10%甲醇+0.1%磷酸水溶液为提取液, 超声提取, 离心去除杂质, 取上清液并用微孔滤膜(0.2 μm)过滤, 以十二烷基硫酸钠(SDS)缓冲液-乙腈(74:26, V:V)为流动相, 利用高效液相色谱分析, 外标法定量。结果 该方法经过验证在0.20~30 mg/L质量浓度范围内, 线性关系良好, 烟酸、烟酰胺相关系数大于0.999, 回收率在99.07%~110.33%之间, RSD 小于5%。结论 与现行标准方法相比, 本方法前处理方法简单、基质干扰小, 测定结果准确度高、重现性好, 尤其对米粉中烟酸及烟酰胺的提取效率更高, 可用于婴幼儿配方米粉和乳粉中烟酸及烟酰胺含量测定的快速、批量分析。

英文摘要:

      Objective To establish a method for the detection of nicotinic acid and nicotinamide in infant formula rice powder and milk powder by high performance liquid chromatography (HPLC). Methods Nicotinic acid and nicotinamide in infant formula powder and milk powder were extracted by 10% methanol and 0.1% phosphoric acid solution. After ultrasonic extraction, the samples were centrifuged to remove impurity. Then the extraction was filtered by syringe filter (0.2 μm), measured by HPLC with sodium dodecyl sulfate (SDS) buffer acetonitrile (74:26, V:V) as mobile phase and external standard method was used for quantification. Results The method showed a good linearity ranged from 0.20 to 30 mg/L of nicotinic acid and nicotinamide concentration with correlation coefficients above 0.999. The recovery was 99.07%~110.33% and RSD was less than 5%. Conclusion Compared with the current standard method, this method is simple, and has low matrix interference, high accuracy, reproducibility, and high extraction efficiency, which is suitable for the rapid and batch determination of nicotinic acid and nicotinamide in infant formula rice powder and milk powder.