实时荧光定量PCR技术在食用油脂检测中的应用

Application of real-time fluorescent quantitative PCR technology in the detection of edible oils

中文关键词:  实时荧光定量PCR  食用油脂  DNA提取

英文关键词:real-time fluorescent quantitative PCR  edible oils  DNA extraction

中文摘要:

      荧光定量PCR检测技术具有快速、准确的优点, 在转基因食品检测等领域得到了广泛的应用。采用荧光定量PCR技术进行油脂转基因、掺假检测也成为研究热点。利用不同油料作物所含有的独特核酸序列, 采用荧光定量PCR技术可简单、高效、快速地检测出油脂中所含的特定核酸成分, 从而判定油脂原料的构成, 为打击食用油脂掺假造假提供判定依据。植物油的加工过程中都经过多个步骤的处理, 其中的核酸降解严重, 含量极低, 所以从植物油中提取出较高质量的DNA是对油脂进行荧光定量PCR检测鉴定的关键。本文主要对油脂DNA提取方法及存在的难点、引物设计特点和结果分析进行了论述, 以期为今后荧光定量PCR检测技术进一步推广与应用提供思路。

英文摘要:

      Fluorescence quantitative PCR technology has been widely used in the detection of genetically modified food and other fields for its quickness and accuracy, and it also becomes popular in transgenic detection and adulteration detection of edible oil. By taking advantage of the unique nucleic acid sequence of different species, the specific nucleic acid composition contained in different oil crops can be detected, and the raw material constituent of edible oil can be predicated, so as to provide the evidence for administrative organization to punish the adulterations of edible oil. The quantity and integrity of DNA of edible oil is very low due to the multiple refined processes. It is critical to get high quality DNA from edible oils for real-time fluorescent quantitative PCR analysis. This article mainly reviewed methods and existing problems of DNA extraction, primer design characteristics and result analysis, so as to provide perspectives for further application of real-time fluorescent quantitative PCR in future.