微生物法测定肠内营养粉剂中微量维生素 B12的含量

Determination of trace vitamin B12 in enteral nutrition powder by microbiological method

中文关键词:  维生素B12  肠内营养粉剂  微生物法

英文关键词:vitamin B12  enteral nutrition powder  microbiological method

中文摘要:

      目的 建立微生物法测定肠内营养粉剂中微量维生素B12的含量。方法 以莱士曼氏乳酸杆菌为实验菌, 在测定用培养基中供给除维生素B12以外的所有营养成分, (36±1) ℃培养19~20 h, 采用比浊法测定。结果 维生素B12在0.001~0.010 ng/mL浓度范围内线性关系良好, 肠内营养粉剂中维生素B12的含量为2.9 μg/100 g。结论 该方法检测结果准确、可靠, 适用于肠内营养粉剂中微量维生素B12的含量测定。

英文摘要:

      Objective To establish a method for the determination of trace vitamin B12 in enteral nutrition powder by microbiological method. Methods The test organism was Lactobacillus leichmamii. The medium without vitamin B12 was used as assay medium. The sample were incubated for 19~20 h at the temperature of (36±1) ℃ and measured by microbial turbidimetric method. Results Vitamin B12 had a good linear relationship in the range of 0.001~0.010 ng/mL, and the content of vitamin B12 in enteral nutrition powder was 2.9 μg/100 g. Conclusion This method is accurate and reliable, and suitable for the assay of trace vitamin B12 in enteral nutrition powder.