高效液相色谱法测定糕点中的栀子黄

Determination of gardenia yellow cake by high performance liquid chromatography

中文关键词:  栀子黄  高效液相色谱法  栀子苷  藏花酸  藏花素

英文关键词:gardenia yellow  high performance liquid chromatography  geniposide  crocetin  crocin

中文摘要:

      目的 改进GB/T5009.149-2003《食品中栀子黄的测定 第一法 高效液相色谱法》的方法。方法 样品经甲醇:甲酸(6:4, V:V)洗脱, 采用 Angilent Extend C18反相色谱柱(4.6 mm×250 mm, 5 μm), 以甲醇:0.2%氨水(50:50, V:V) 为流动相(等度洗脱), 流速为1.0 mL/min, 进样量20 μL, 经配有二极管阵列检测器(DAD)的高效液相色谱仪(high performance liquid chromatograph, HPLC)进行测定。结果 本方法检出限为3.2 mg/kg, 栀子黄中藏花酸和藏花素峰面积的相对标准偏差分别为1.6%、2.5%, 在5~50 μg/mL范围内有较好的线性, Y=1.34X-0.54, r2=0.9998, 不同浓度的回收率在91.2%~98.4%之间。结论 本方法简单、高效、准确度高, 回收率和重现性良好, 适用检测的食品类别范围较宽, 具有推广应用价值。

英文摘要:

      Objective To improve the method of GB/T 5009.149-2003 Determination of crocin in food by high performance liquid chromatography. Methods After eluted with methanol:formic acid (6:4, V:V), samples were determined by high performance liquid chromatography (HPLC) equipped with diode array detection (DAD), using Agilent extend C18 reversed-phase chromatographic column (4.6 mm×250 mm, 5 μm), with methanol: 0.2% ammonia water (50:50, V:V) as mobile phase (isocratic elution) at a flow rate of 1.0 mL/min, and injection volume of 20 μg. Results The detection limit of the method was 3.2 mg/kg. The RSDs of the peak area of crocetin and crocin in gardenia yellow were 1.6% and 2.5%, respectively. And they had a good linearity in the range of 5~50 μg/mL, Y=1.34X-0.54, r2=0.9998. The recoveries were between 91.2%~98.4%. Conclusion This method is simple, efficient, accurate, and has good recovery rate and reproducibility, which is suitable for the detection of a wide range of food categories, and has the value of popularization and application.