乙酰丙酮法测定啤酒中甲醛含量的研究

Determination of the formaldehyde content in beer by acetyl acetone method

中文关键词:  甲醛  啤酒  乙酰丙酮法

英文关键词:formaldehyde  beer  acetyl acetone method

中文摘要:

      目的 改进乙酰丙酮法测定啤酒中甲醛含量。方法 采用乙酰丙酮比色法测定啤酒中甲醛含量。通过3组加标回收试验, 测定不同蒸馏体积、不同反应时间、不同磷酸浓度对乙酰丙酮显色法的干扰, 确定最合适的检测条件。结果 最佳条件为: 蒸馏液为200 mL, 加入硫酸浓度为20%, 反应时间5 min。甲醛含量在0~50 μg范围内具有良好的线性关系, 线性方程为Y=0.022X-0.001, r2=1, 回收率大于80%。结论 该方法用于检测啤酒中甲醛含量简单、快速、准确, 易于推广。

英文摘要:

      Objective To improve the acetyl acetone colorimetric method for the determination of formaldehyde in beer. Method The formaldehyde content in beer was detected by acetyl acetone colorimetric method. The optimal experimental conditions were determined by 3 groups of spiked recovery tests, through manipulating the different distillation volumes, different reaction times and different concentrations of phosphate to interfere acetylacetone colorimetric method. Results The optimal conditions for the experiment were as follow: the distillate was 200 mL, the sulfuric acid concentration was 20% and the reaction time was 5 min. The formaldehyde content had a good linear relationship in the range of 0~50 μg, and the linear equation was Y=0.022X-0.001 (r2=1). The recovery was more than 80%. Conclusion The method for detecting formaldehyde content in beer is simple, rapid, accurate and easy to disseminate.