高效液相色谱法同时测定可乐中苯甲酸、糖精钠、安赛蜜和咖啡因

Simultaneous determination of benzoic acid, sodium saccharin, acesulfame potassium and caffeine in cola soft drink by high performance liquid chromatography

中文关键词:  苯甲酸  糖精钠  安赛蜜  咖啡因  可乐  高效液相色谱法  FAPAS

英文关键词:benzoic acid  sodium saccharin  acesulfame potassium  caffeine  cola  high performance liquid chromatography  FAPAS

中文摘要:

      目的 建立高效液相色谱同时测定可乐中苯甲酸、糖精钠、安赛蜜和咖啡因的方法, 并应用于国际FAPAS能力验证。方法 样品经超声脱气用水稀释, 采用C18色谱柱分离, 以乙酸铵缓冲液(0.02 mol/L)/甲醇为流动相, 采用梯度洗脱分离, 在230 nm、272 nm波长下进行检测。结果 苯甲酸、糖精钠、安赛蜜和咖啡因线性范围为2.0~200 μg/mL, 线性关系良好, 相关系数大于0.999, 回收率在97%~103%之间, RSD为0.5%~2.8%之间, 检出限在0.005~0.02 μg/mL之间。结论 该法灵敏度高、重现性好, 用于FAPAS能力验证时结果接近中值。

英文摘要:

      Objective To establish a methodfor the simultaneous determination of benzoic acid, sodium saccharin, acesulfame potassium and caffeine in cola by high performance liquid chromatography (HPLC), and apply to international FAPAS proficiency testing. Methods After ultrasonic degasification, the samples were di-luted by water, separated on a C18 column with the mobile phase of ammonium acetate (0.02 mol/L)/methanol in gradient elution, and detected at the wavelength of 230 nm and 272 nm. Results The calibration curves within the range of 2.0~200 μg/mL showed a good linearity and the correlation coefficients were higher than 0.999. The individual average recovery at 3 spiked concentration levels ranged from 97% to 103%, while the relative standard deviations (RSD) were between 0.5%~2.8% (n=6). The limits of detection of benzoic acid, sodium saccharin, acesulfame potassium and caffeine were 0.005~0.02 μg/mL. Conclusion Theestablished method is sensitive and accurate and can be used to the FAPAS proficiency testing, and theZ-scores of the benzoic acid, saccharin, acesulfame potassium and caffeine were close to median.