豆浆中胰蛋白酶抑制剂活性测定方法的研究

Determination of trypsin inhibitor activity of soybean milk

中文关键词:  豆浆  胰蛋白酶抑制剂活性  测定方法

英文关键词:soybean milk  trypsin inhibitor activity  determination method

中文摘要:

      目的 在GB/T 21498-2008《大豆制品中胰蛋白酶抑制剂活性的测定》方法的基础上, 对影响豆浆中胰蛋白酶抑制剂活性测定的一些关键因素进行研究和改进。方法 豆浆样品加水提取并稀释至一定体积后, 加入L-BAPA试剂和胰蛋白酶使用液, (37±0.25) ℃水浴中保温10 min±5 s后, 加入乙酸溶液终止酶反应, 并采用分光光度计在410 nm波长下测定其吸光度值, 代入公式计算胰蛋白酶抑制剂的活性。结果 改进后的方法添加高、中、低3个浓度水平胰蛋白酶抑制剂的回收率分别为113.1%、114.6%、134.9%, 平行测定的RSD分别为1.4%、3.6%、7.2%, 方法的准确性和重复性能满足实验的需求。结论 改进后的方法快速简便, 节约了时间和实验成本, 方法的准确性和重复性能满足实验的需求。

英文摘要:

      Objective To study and improve some key factors influencing determination of trypsin inhibitor activity of soybean milk, based on the method of GB/T 21498-2008 Determination of trypsin inhibitor activity of soya products. Methods Soybean milk samples were extracted by water and diluted to a certain volume, with adding L-BAPA reagent and trypsin using liquid, and (37+0.25) ℃ water bath insulation for 10 min±5 s, then the enzyme reaction was ceased by adding acetic acid. The absorbance value was determined at the wavelength of 410 nm by spectrophotometer and the trypsin inhibitor activity was calculated by substituting into the formula. Results The average recoveries of low, medium and high concentrations of standard samples were 113.1%, 114.6% and 134.9%, and the RSDs of parallel determination were 1.4%, 3.6% and 7.2%, respectively. The accuracy and reproducibility of the method has met the satisfaction of the experiment. Conclusions The improved method is time-saving and low-cost, and had high accuracy and precision, which also meets the requirements of the experiment.