高效液相色谱-柱后衍生法测定植物油脂中 4种黄曲霉毒素

Determination of four kinds of aflatoxins in vegetable oil by high performance liquid chromatography with post column derivation

中文关键词:  高效液相色谱  黄曲霉毒素  免疫亲和柱  植物油脂

英文关键词:high performance liquid chromatography, aflatoxins, immunoaffinity column, vegetable oil

中文摘要:

      目的 建立一种可同时测定植物油脂中黄曲霉毒素G2、G1、B2、B1残留量的分析方法。方法 将待测样品经前期溶剂提取、免疫亲和柱净化处理后, 通过高效液相色谱-柱后衍生-荧光检测器进行测定。结果 黄曲霉素G2、B2在0.1~2 ng/mL范围内表现出良好的线性关系, 而黄曲霉素G1、B1在1~20 ng/mL范围内r>0.9995, 其线性关系良好。在添加浓度为0.1~20 μg/kg的范围内, 平均回收率在75.0%~125.7%之间。结论 该方法快速简便准确, 可作为植物油脂中黄曲霉毒素的残留量测定方法。

英文摘要:

      Objective To establish a simultaneous detection method for the determination of aflatoxin G2, G1, B2 and B1 residue in vegetable oil. Methods After solvent extraction and purification with immunoaffinity column, the oil samples were analyzed by high performance liquid chromatography-post column deriva-tion-fluorescence detector (HPLC-PCD-FLD). Results The linear relationship of aflatoxin G2 and B2 ranged from 0.1~2 ng/mL, and of aflatoxin G1 and B1 ranged from 1~20 ng/mL was excellent, and r was larger than 0.9995. The average recovery rate was from 75.0% to 125.7% when the addition concentration ranging from 0.1 μg/kg to 20 μg/kg. Conclusion This method is simple and quick, and can be used for the determination of aflatoxins residue in vegetable oil.