食品能力验证中沙门氏菌的分离和鉴定

Isolation and identification of Salmonella spp. in food during the proficiency testing

中文关键词:  能力验证  沙门氏菌  菌株分离  菌株鉴定

英文关键词:proficiency testing  Salmonella  strain isolation  strain identification

中文摘要:

      目的 为了提升实验室食品中沙门氏菌检测能力, 增强实验室竞争能力, 本实验室参加了中国食品药品检定研究院组织的沙门氏菌检测能力验证活动。方法 依据中华人民共和国国家标准GB 4789.4-2010, 采用传统分离方法和血清学鉴定, 联合全自动微生物生化鉴定系统(VITEK2-compact)对分离出的疑似菌进行生化鉴定。结果 编号为CODE1样品+CODE1奶粉混合样检出纽波特沙门氏菌和科林德尔沙门氏菌, CODE3样品+CODE3奶粉混合样检出维普拉沙门氏菌、鼠伤寒沙门氏菌和埃科沙门氏菌, 其余8个样品未检出。结论 顺利完成本次能力验证活动。

英文摘要:

      Objective To strengthen the detection capability of Salmonella in food and improve the competition ability of laboratory, the laboratory participated in proficiency testing on Salmonella detection in foods by National Institutes for Food and Drug Control. Methods Traditional separation and serological identifica-tion methods were carried out according to GB 4789.4-2010. The biochemical identification of isolated suspicious colonies was detected by biochemical identification system (VITEK2-compact). Results Salmonella newport and Salmonella colindale were detected in the mixed sample of CODE 1 and CODE 1 powdered milk, Salmonella wipprat, Salmonella typhimurium and Salmonella eko were detected in the mixed sample of CODE 3 and CODE 3 powdered milk, but Salmonella spp. were not detected in other 8 samples. Conclusion The proficiency testing was successfully completed.