食源性致病菌免疫学检测方法研究进展

Research progress of immunological detection methods of foodborne pathogen

中文关键词:  食源性致病菌  免疫学方法  快速检测

英文关键词:foodborne pathogens  immunological method  rapid detection

中文摘要:

      食源性致病菌广泛存在, 因其能引起人的疾病甚至死亡, 而越来越受世界各国的关注。食源性致病菌传统检测方法为培养法, 其费时耗力, 不适用于快速检测。本文介绍的免疫学方法具有方便、快速等优点, 因而越来越被广泛应用。免疫学检测方法主要有酶联免疫吸附法、免疫层析法、免疫磁珠分离技术、化学发光免疫分析法和乳胶凝集法等。本文着重对这些方法的原理及其应用进行综述, 阐述其优缺点, 同时对免疫学检测方法未来的发展趋势进行了展望。

英文摘要:

      Foodborne pathogen widely exists and has drawn more and more attention as it can cause human disease even to death. Traditional detection method of foodborne pathogens is very time-consuming and cannot meet the need of rapid detection. Immunological detection methods are convenient and rapid, including enzyme-linked immunosorbent assay, immunochromatography, immunomagnetic beads separation techniques, chemiluminescence immunoassay and latex agglutination assays. In this paper, the principle, application, advantages, and disadvantages of these methods were reviewed. The future development trend of the immunological detection method was prospected.