大孔树脂吸附纯化番木瓜籽总三萜研究

Study on purification of total triterpenes from papaya seeds with macroreticular resins

中文关键词:  番木瓜籽  总三萜  大孔树脂  纯化

英文关键词:papaya seeds  total triterpenes  macroporous resin  purification

中文摘要:

      目的 筛选出适宜吸附纯化番木瓜籽总三萜的树脂类型, 初步确定其吸附-解析工艺条件。方法 采用S-8、NAK-9、AB-8、HPD700和D-101 5种不同极性大孔树脂对番木瓜籽总三萜进行吸附纯化, 通过比较5种树脂对番木瓜籽总三萜的吸附解析性能及产品回收率, 筛选出最佳树脂类型及确定吸附纯化工艺。结果 试验表明, 相较其他4种树脂, 弱极性树脂AB-8较适用于番木瓜籽总三萜的吸附纯化, 建立了AB-8静态吸附总三萜的动力学方程, 确立了AB-8纯化总三萜的工艺条件, 对树脂AB-8的等温吸附进行初步探讨。结论 在洗脱剂乙醇体积分数为80%、吸附2 h、解析2 h, AB-8大孔树脂对番木瓜籽总三萜纯化效果较佳, 产品的解析率达70.89%, 回收率达到59.58%。

英文摘要:

      Objective To filtrate the best macroreticular resins for purification of total triterpenes in papaya seeds and to confirm the technological parameters. Methods The purification of total triterpenes from papaya seeds with 5 types of macroporous resins S-8, NAK-9, AB-8, HPD700, and D-101 was studied. The optimal resin and parameters for purification were confirmed by comparing the properties of adsorption and desorption and recovery. Results Compared to other resins, AB-8 was the most appropriate resin for the purification of papaya seeds total triterpenes. The static adsorption kinetics equation of total triterpenes of AB-8 had been established. Conclusion Validation experiments indicated that with ethanol desorption agent concentration of 80%, 2 h adsorption and desorption 2 h, desorption ratio and product purity could reach 70.89% and 59.58%, respectively.