基于高压脉冲电场技术提高松子肽的抗氧化活性研究

Improvement on antioxidative activity of pine nut peptide treated by pulsed electric field

中文关键词:  高压脉冲电场  松子肽  DPPH自由基清除能力

英文关键词:pulsed electric field  pine nuts peptide  DPPH radical scavenging capacity

中文摘要:

      目的 为了初步揭示高压脉冲电场(PEF)技术提高分子量小于1 kDa松子肽的抗氧化活性的作用机制。方法 以DPPH自由基清除能力为衡量指标, 利用单因素和响应面试验设计研究方法, 构建出基于PEF技术提高分子量小于1 kDa松子肽的抗氧化活性回归模型, 优化出最佳技术参数, 并借助傅立叶红外光谱技术分析其官能团变化情况。结果 回归模型为: Y=84.4+0.31X1+0.6X2+1.13X3-0.26X1X2-0.062X1X3-0.79X2X3-3.32X12-5.21 X22-3.37X32, 最佳技术参数为电场场强12.6 kV/cm、电场频率1616 Hz、物料流速3.456 mL/min, 其DPPH自由基清除能力为83.51?Ee0.61%, 与未经高压脉冲电场处理松子肽相比, 增加了26.69%; 借助傅立叶红外光谱技术分析发现, 分子量小于1 kDa松子肽经PEF处理后的?C≡N键发生变化。结论 高压脉冲电场技术可以提高松子肽的抗氧化活性, 通过傅立叶红外光谱技术可知抗氧化活性的提高可能是由于松子肽的?C≡N键发生变化。

英文摘要:

      Objective To reveal antioxidative mechanism of pine nut peptides preliminarily by pulsed electric field (PEF) technology, the molecular weight of pine nut peptides was less than 1 kDa. Methods The DPPH radical scavenging capacity was used to measure in the study, the methods of single factor and response surface experiment were designed to build the regression model of pine nuts peptides, which were treated by pulsed electric field (PEF). And the optimal results were acquired by optimizing. The functional group changes were analyzed by fourier infrared spectroscopy. Results The regression model was: Y=84.4+0.31X1+0.6X2+ 1.13X3-0.26 X1X2-0.062X1X3-0.79X2X3-3.32X12-5.21X22-3.37X32, and the optimal results were: field intensity of 12.6 kV/cm, frequency of 1616 Hz, flow rate of 3.456 mL/min, and the DPPH radical scavenging capacity was 83.51?Ee0.61%. Compared with untreated pine nut peptides, it was increased by 26.69%. Fourier infrared spectroscopy was used to analyze pine nut peptides which the molecular weight was less than 1 kDa. The ?C≡N was changed when it treated by PEF. Conclusion Pulsed electric field technology can improve the antioxidant activity of pine nuts peptide. The increase of antioxidantive activity might be due to the changes of ?C≡N bonding by fourier infrared spectroscopy.