黑米花青素微胶囊制备的工艺研究

Study on preparation of microcapsules of anthocyanins from black rice

中文关键词:  花青素  缓释  微胶囊  益生元  工艺研究

英文关键词:anthocyanins  sustained release  microcapsule  prebiotics  technology research

中文摘要:

      目的 以黑米花青素为主要原料, 开发一种能够顺利到达肠道并在肠道内缓慢释放以发挥花青素益生元功效的微胶囊。方法 采用锐孔法, 以包埋率为指标, 分别以壁芯比、针头孔径、海藻酸钠及CaCl2浓度为因素, 采用单因素试验, 确定微胶囊的制备条件; 在此基础上, 采用四因素三水平L9(34)正交试验, 优化了该微胶囊的最佳制备工艺; 最后, 通过微胶囊在胃和肠模拟液中的释放试验, 验证该微胶囊的缓释功能。结果 微胶囊的最佳制备工艺: 壁芯比3:1、针头孔径0.45 mm、海藻酸钠2.0%、CaCl2 2.0%; 该微胶囊可以在人胃模拟液中保持4.0 h以上, 在人肠模拟液中缓慢释放。结论 采用锐孔法制备的花青素微胶囊能够顺利到达肠道缓慢释放, 发挥其益生元功效。

英文摘要:

      Objective To develop a kind of microcapsule of anthocyanins from black rice, which can arrive to the intestinal successfully with a sustained release and exert the prebiotic efficacy of anthocyanins. Methods Using the orifice method, the imbedding ratio of anthocyanins as evaluation index and the wall-core ratio, aperture of needle, sodium alginate mass fraction, and CaCl2 concentration as factors, the conditions of making microcapsule were determined by single-factor experiments. On this basis, the preparation technology was optimized by orthogonal experiment. At last, the sustained-release function of microcapsules was verified by releasing experiment in the human stomach and intestine simulation liquid. Results The optimum preparation process was as follow: wall-core ratio 3:1, aperture of needle 0.45 mm, sodium alginate 2.0% and CaCl2 2.0%. The microcapsules could maintain in the stomach simulation liquid for 4.0 h or more, and release slowly in the intestine simulation liquid. Conclution The microcapsules made with the orifice method could reach the intestinal canal successfully, release slowly and exert the prebiotics efficacy of anthocyanins.