气相色谱-串联质谱法检测水产品中氯霉素残留量

Determination of chloramphenicol residues in aquatic products by gas chro-matography-tandem mass spectrometry

中文关键词:  氯霉素  气相色谱-串联质谱法  药物残留  水产品

英文关键词:chloramphenicol  gas chromatography-tandem mass spectrometry/mass spectrometry  drug residues  aquatic products

中文摘要:

      目的 建立检测分析水产品中氯霉素残留量的气相色谱-串联质谱联用(GC-MS/MS)的方法。方法 样品采用乙酸乙酯提取、经过C18固相萃取柱净化, 用硅烷化试剂衍生化, 采用GC-MS/MS分析时, 氯霉素在15 min内流出。结果 对样品添加两种浓度的加标质控, 回收率在80%以上, 相对标准偏差(RSD)在6.2%以内。在0.5~10 mg/L质量浓度范围内呈线性关系, 相关系数大于0.99。结论 该方法可用于氯霉素残留的准确定量分析, 灵敏度、精密度和准确度均能满足渔药残留检测分析要求。

英文摘要:

      Objective To establish a determination method of chloramphenicol (CAP) in aquatic products by gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The samples were extracted by ethyl acetate, cleaned up by C18 SPE column, and derived with silanization reagent, then the extracts were de-termined by MS/MS detector based on SCAN and MRM mode. Results CAP was well separated within 15 min. This method showed that the spiked average recoveries above 80%, the RSD ranged within 6.2%, and the calibration of those pesticides were linearity within the range of 0.5～10 mg/L (r2>0.99). Conclusion The method is accurate and sensitive, it is suitable for analysis of CAP residues in aquatic products.