高效液相色谱法测定保健食品中三氯蔗糖的含量

Determination of sucralose content in health food by high performance liquid chromatography

中文关键词:  高效液相色谱法  三氯蔗糖  保健食品  含量测定

英文关键词:high performance liquid chromatography  sucralose  health food  content determination

中文摘要:

      目的 建立一种保健食品中三氯蔗糖含量测定的高效液相色谱检测法。方法 样品采用Rad pak C18色谱柱(4.6 mm×250 mm, 5 μm)进行分离, 以1.0 mL/min乙腈:水(V:V=15:85)为流动相, 最后用高效液相色谱法(HPLC)-示差折光仪为检测器进行测定, 外标法定量分析。结果 高效液相色谱法在0.482~2.892 mg/mL浓度范围, 三氯蔗糖的浓度和峰面积的线性良好, 相关系数为0.999(r＞0.99), 该方法的检出限和定量限分别为19.58 mg/g和65.27 mg/g, 在不同的添加水平下, 该方法的回收率范围为95.0%~95.6%, 相对标准偏差为0.3% (n=9)。结论 高效液相色谱法灵敏度、准确度均较高, 适用于保健食品中三氯蔗糖的含量测定。

英文摘要:

      Objective To establish an efficient solution for determination of sucralose content in health food by high performance liquid chromatography (HPLC). Methods The sample was separated on a Rad Pak C18 chromatographic column (4.6 mm×250 mm, 5 μm), using 1 mL/min acetonitrile:water (V:V=15:85) as the mobile phase. Then, the analyte was detected by HPLC with different refractometer detector and external standard method for quantitative analysis. Results The method showed a good linearity in the range of 0.482~2.892 mg/mL with r=0.999(>0.99), and the detection limit and quantification limit were 19.58 and 65.27 mg/g respectively. Under different additive levels, the recovery rate of this method was ranged from 95.0% to 95.6%, the relative standard deviation (RSD) was 0.3% (n=9). Conclusion This method is suitable for sensitive and accurate determination of sucralose in health food.