一株高产D-阿拉伯醇菌株的分离与筛选

Isolation and screening of a strain for high D-arabitol production

中文关键词:  D-阿拉伯醇  葡萄糖  酵母菌  筛选  鉴定

英文关键词:D-arabitol  Glucose  Yeast  Screening  Identification

中文摘要:

      目的 分离和筛选出一株高产D-阿拉伯醇菌株。方法 采用初筛和复筛手段, 利用纸层析法和高效液相检测方法, 从具有高浓度葡萄糖环境, 如葡萄干、红薯地土壤、蜂蜜、花粉等含糖量较高的样品中分离和筛选得到了一株高效转化葡萄糖生成D-阿拉伯醇的优势菌株。结果 该菌株在初始葡萄糖浓度为200 g/L 的培养基中, 30 ℃, 摇瓶培养120 h, D-阿拉伯醇的产量高达82.4 g/L。根据常规形态鉴别, 生理生化实验测定, 以及核酸序列同源性比较分析, 证实筛选出的高产菌株b-1属于Candida parapsilosis。结论 优势菌株b-1发酵产D-阿拉伯醇的产率较高, 副产物较少, 具有一定的工业化应用前景。

英文摘要:

      Objective To isolate and screen a high yield strain for D-arabitol production. Methods With the determination of paper chromatography and high performance liquid chromatography (HPLC), the fine strain for high concentration yield of D-arabitol from glucose had been separated and screened from high glucose environment, such as raisins, sweet potato to soil, honey, pollen samples, after the first and second screening. Results At the initial glucose concentration of 200 g/L on the culture medium, 30 ℃, D-arabitol yield was 82.4 g/L after the fermentation of 120 h. According to the conventional morphological identifica-tion, determination of physiological and biochemical experiment, and the nucleic acid sequence homology comparison analysis, the high-producing strain b-1 was confirmed as Candida parapsilosis. Conclusion The yield of D-arabitol is high, the by-products are low in the fermentation by the screened strains b-1, which has a certain application prospect of industrialization.