高效液相色谱-串联质谱法测定草莓中5种 植物生长调节剂

Determination of 5 kinds of plant growth regulators in strawberry by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  植物生长调节剂  QuEChERS  高效液相色谱-串联质谱法

英文关键词:plant growth regulators  QuEChERS  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立高效液相色谱-串联质谱法(HPLC-MS/MS)检测草莓中3-吲哚丁酸、赛苯隆、氯吡脲、3-吲哚乙酸、脱落酸5 种植物生长调节剂的方法。方法 样品采用QuEChERS方法乙腈提取, PSA吸附剂净化, 高效液相色谱-串联质谱电喷雾(ESI), 多反应监测(MRM)负离子模式分析检测, 外标法定量。结果 各化合物在0.01~1.0 mg/L范围内均呈现良好的线性关系, 相关系数均大于0.998。5种化合物的检出限在1~5 μg/kg。添加回收率为72.0%~99.4%。相对标准偏差(RSD)均小于10%。结论 通过线性、准确度、精密度实验及样品测定, 验证了以水溶解样品、乙腈提取、QuEChERS净化的方法简便可行。

英文摘要:

      Objective To establish a method for the determination of 5 kinds of plant growth regulators (PGRs): 4-(3-Indolyl)-butyric acid (BAA), thidazuron, forchlorfenuron (CPPU), 3-Indolyl-acetic acid (ZAA) and abcisic acid (ABA) in food of plant origin using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods Samples were extracted by acetonitrile, and cleaned up by QuEChERS. Identification and determination were achieved by multiple reaction monitoring in negative mode using HPLC-MS/MS. The quantification of analytes was carried out by the external standard method. Results All the target compounds had a good linear relationship in the range of 0.01~1.00 mg/L. The relative coefficients were larger than 0.998. The LOQ of the tested analytes varied was between 1~5 μg/kg. The recoveries were 72.0%~99.4%. The relative standard deviations (RSD) were less than 10%. Conclusion The method was proved to be simple and accurate in practice.