水提紫甘薯色素废渣总黄酮纯化工艺研究

Study on purification of total flavonoids from residue of water-extracting pigment of Ipamoea batatas L.

中文关键词:  水提紫甘薯色素废渣  黄酮  纯化  工艺

英文关键词:residue of water-extracting pigment of Ipamoea batatas L.  flavonoids  purification  process

中文摘要:

      目的 在前期对水提紫甘薯色素废渣总黄酮提取研究基础上, 本研究进一步深入研究其总黄酮的纯化工艺, 旨在为水提紫甘薯色素废渣的综合开发利用提供理论基础和参考依据。方法 通过静态吸附、解吸和动态吸附、解吸等试验来考察AB-8大孔树脂的纯化性能, 对水提紫甘薯色素废渣总黄酮粗提液进行纯化。结果 AB-8大孔树脂对水提紫甘薯色素废渣总黄酮有较好的吸附和解吸性能, 吸附率达86.43%; 最佳上样pH值为3.0; 解吸液以2BV浓度为80%的乙醇水溶液解吸效果最好, 解吸率达89.79%; 解吸流速以1 mL/min效果最好。结论 采用AB-8大孔吸附树脂纯化水提紫甘薯色素废渣总黄酮所得工艺具有较好的纯化效果, 且方法简便可行。

英文摘要:

      Objective The study on purification of the total flavonoids from residue of water-extracting pigment of Ipamoea batatas L. aimed to provide the theoretical basis and reference for its comprehensive development and utilization. Methods The depurated performance of AB-8 macroporous adsorption resin was investigated through static adsorption and desorption, dynamic adsorption and desorption experiment. Then the crude extraction of total flavonoids from residue of water-extracting pigment of Ipamoea batatas L. was purified. Results The AB-8 macroporous resin pigment purple sweet potato waste water extraction of total flavonoids have better absorption and desorption properties. The adsorption rate of total flavonoids was 86.43%, respectively. The pH of solution was 3.0 for total flavonoids. The optimum desorption process was desorpted at 1 mL/min with 2BV of 80% ethanol, and the desorption rate of total flavonoids was 89.79%, respectively. Conclusion The technology that adopts the AB-8 macroporous adsorption resin which purifies the total flavonoids from residue of water-extracting pigment of Ipamoea batatas L. has a good purification effect, which is also simple and feasible.