高效液相色谱—串联质谱法快速测定胶原蛋白粉中呋喃类代谢物

Rapid determination of nitrofuran metabolite residues in collagen powder by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  呋喃类代谢物兽药残留  液相色谱—串联质谱法  胶原蛋白粉  乳化现象

英文关键词:nitrofuran metabolite residues of veterinary drugs  liquid chromatography-tandem mass spectrometry  collagen powder  emulsification

中文摘要:

      目的 简化测定胶原蛋白粉中呋喃类代谢物残留的实验过程, 缩短实验的检测时间。方法 在GB/T21311-2007的基础上, 对其进行方法改进, 建立高效液相色谱-串联质谱法(HPLC-MS/MS)测定胶原蛋白粉中呋喃西林(SEM)、呋喃妥因(AHD)、呋喃唑酮(AOZ)、呋喃它酮(AMOZ)等4种呋喃代谢物的快速检测方法。采用55 ℃衍生化, 对衍生品按照离心、调pH值、离心的步骤进行处理, 使得调节pH值更容易, 同时避免传统试验方法中使用分液漏斗时样品乳化成胶状体而不能达到液液分离的目的的现象。结果 实验结果表明, 本方法与《GB/T21311-2007动物源性食品中硝基呋喃类药物代谢物残留量检测方法高效液相色谱串联质谱法》相比, 结果并无很大的差异, 但是大大缩短了实验的检测时间、简化了实验过程。结论 本实验方法更加适合大批量样品的试验检测。

英文摘要:

      Objective To simplify the experimental process for determination of furan metabolite residues in collagen powder, and shorten the detection time of the experiment. Methods Based on GB/T21311-2007, the method was improved, and a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to rapidly determine 4 kinds of nitrofuran metabolite residues in collagen powder, including nitrofurazone (SEM), nitrofurantoin (AHD), furaltadone (AOZ) and furazolidone (AMOZ). After derivation at 55 ℃, the derivatives were processed following the steps of centrifugation, pH adjustment, and centrifugation again, making it easier to adjust the pH value, while avoiding the problem found in the conventional test method using a separatory funnel where the sample emulsified into colloid and could not achieve the purpose of liquid-liquid separation. Results The experimental results showed no significant differences between the proposed method and the national standard GB/T21311-2007, while the detection time of the experiment was greatly reduced, and the experimental process was simplified. Conclusion This method is more suitable for experimental detection of large quantities of samples.