保健食品中非法添加药物的检测现状及筛查策略研究

Detection status and screening strategy of illegally added drugs in healthy food

中文关键词:  信息依赖型策略  保健食品  非法添加药物  筛查策略

英文关键词:information-dependent strategy  healthy food  illegally added drug  screening strategy

中文摘要:

      目的 为保健食品中非法添加药物的发现提供筛查策略和方法, 以弥补现有检测方法的不足。方法 首先针对可以实现或者辅助实现保健食品声称功能的药物建立了对应的信息库, 对其主要临床功效和副作用进行了信息汇总。其次通过质谱单离子扫描和子离子扫描, 建立上述物质的质谱筛查信息。同时按照不同能量水平, 建立不同信号强度的质量信息库。进而作为主体信号, 建立液相质谱多反应检测的方法, 及其液质联用采集信息, 并选择了不同剂型、不同基体的保健食品作为基质, 建立了阴性、添加阳性的数据信息库。形成了治疗信息、分析检测信号及确证信息为线索的筛查体系和方式。结果 以缓解体力疲劳类药物为例, 通过对近百批相关保健食品进行筛查, 可见阳性结果发现率较一般方法提高了100%, 而对于未知物质的发现提高了300%, 验证了以信息依赖型为筛查主线的方法策略。结论 信息依赖型保健食品非法添加药物筛查为保健食品中非法添加药物的发现提供了新的途径。

英文摘要:

      Objective To provide screening strategy and method for the discovery of illegally added drugs in healthy foods. Method Firstly, based on the drugs which could realize or help to realize the promised function of health food, the corresponding database was established, which was aimed at collecting main clini-cal efficacy and side effects of drugs. Secondly, through scanning of mass spectrometry single ion and prod-uct ion, the screening information of mass spectrometry was founded. Meanwhile, according to the different energy levels, database based on different signal intensities was set up. Based on the above methods, liquid chromatography-mass spectrometry (LC-MS) multi-reactions monitoring method was applied and LC-MS information was collected. By choosing health food of different dosage forms and substrates, negative data and added positive data were included in the database. Thus, the screening system and method were formed in terms of therapy information, the analysis of test signals and confirmed information. Results The information-dependent method was validated by screening function to relieve physical fatigue drugs as example. According to the screening of hundreds batches of relevant health food, the discovery rate of positive results increased by 100% compared to the general methods and that of unknown substances risen by 300%. Conclusion The information-dependent strategy for illegally added drug in healthy food provided a novel path for control of the quality of healthy food.