高效液相色谱-串联质谱法同时测定婴幼儿配方 食品中香兰素、甲基香兰素和乙基香兰素

Simultaneous determination of vanillin, methyl vanillin and ethyl vanillin in infant formulas by high performance liquid chromatography-tandem mass spectrometry method

中文关键词:  婴幼儿配方食品  香兰素  甲基香兰素  乙基香兰素  高效液相色谱-串联质谱法

英文关键词:infant formula  vanillin  methyl vanillin  ethyl vanillin  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立了高效液相色谱-串联质谱法同时测定婴幼儿食品中香兰素、甲基香兰素和乙基香兰素的方法。方法 样品经Tris溶液和乙腈振荡提取, 离心后提取液用正己烷脱脂过滤后上机测定, 外标法定量。 结果 3种化合物在5.0~200 μg/L范围内线性良好, 在液态奶中测定低限均为25 μg/kg, 在奶粉中测定低限均为50 μg/kg, 方法加标收率在89.5%~115%之间, 相对标准偏差(RSD)小于15.6%。结论 该方法简便、快速, 准确, 灵敏度及精密度高, 适用于婴幼儿食品中香兰素、甲基香兰素和乙基香兰素的测定。

英文摘要:

      Objective A method is presented for the quantitative determination and confirmation of vanillin, methyl vanillin and ethyl vanillin in infant formulas by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods Samples were extracted by Tris solution and acetonitrile. The supernatant was centrifuged and defatted by n-hexane before the 0.22 μm filter apparatus was used, finally detected by HPLC/MS/MS and quantitated by external standard method. Results A good linearity of three compounds was obtained in the range of 5.0~200 μg/L. The limits of detection of three compounds were 25 μg/kg in liquid milk and 50 μg/kg in milk powder. Mean recoveries of three compounds ranged from 89.5% to 115%. The relative standard deviation was less than 15.6%. Conclusion The method was proved to be a simple, rapid, accurate and sensitive method and was successfully applied to detect vanillin, methyl vanillin and ethyl vanillin in infant formula.