在线超滤-离子色谱法检测鱼子酱中的多聚磷酸盐

Determination of polyphosphates in caviar using ion chromatography-inline ultrafiltration

中文关键词:  离子色谱法  在线超滤  多聚磷酸盐  鱼子酱

英文关键词:ion chromatography  inline ultrafiltration  polyphosphates  caviar

中文摘要:

      目的 建立在线超滤—离子色谱法检测鱼子酱中多聚磷酸盐的方法。方法 利用超纯水超声提取鱼子酱中的多聚磷酸盐, 经在线超滤装置自动在线净化样品, 由电导检测器检测样品中的多聚磷酸盐含量。 结果 该方法检出限(LOD)为10 mg/kg。标准加入法测定所有被测物的高、中、低三个浓度加标水平的回收率范围分别为正磷酸盐95%~120%、焦磷酸盐68%~75%、三聚磷酸盐85%~88%、三偏磷酸盐100%~107%, 相对标准偏差(RSD)在0.38%~1.36%之间。4种被测物在1~80 μg/mL范围内线性良好, R2均大于 0.999。结论 该方法稳定、快速、精密度高、线性范围宽, 适合鱼子酱中多聚磷酸盐的含量测定。

英文摘要:

      Objective To establish a method for the determination of polyphosphates in caviar using ion chromatography-inline ultrafiltration. Methods The polyphosphates in caviar was determined by ion chromatography with inline ultrafiltration, and the conductivity detector was be used to detect polyphosphates in caviar. Results The method detection limit was 10 mg/kg. Recoveries of orthophosphate, pyrophosphate, tripolyphosphate and three metaphosphate were between 95%~120%, 68%~75%, 85%~88% and 100%~107%, respectively. The precision RSD were in the range of 0.38%~1.36%. The lineal range in 1~80 μg/mL was with high correlation coefficients (R2>0.999). Conclusion The method is sensitive, accurate and reproducible, and it is suitable for the determination of polyphosphates in caviar.