柱前衍生RP-HPLC测定杨树花中氨基酸含量

Determination of amino acids in Flos Populi Tomentosae by reversed phase high performance liquid chromatography

中文关键词:  杨树花  氨基酸  反相高效液相色谱法  柱前衍生化

英文关键词:Flos Populi Tomentosae  amino acids  reversed phase high performance liquid chromatography  pre-column derivation

中文摘要:

      目的 建立一种采用柱前衍生-反相高效液相色谱法检测杨树花中游离氨基酸和水解氨基酸含量的方法。方法 以异硫氰酸苯酯进行柱前衍生, 采用高效液相色谱法检测杨树花中游离氨基酸和水解氨基酸含量。结果 21种氨基酸线性范围为0.0019~2.7 μmol?mL-1, R2均＞0.9990; 21种游离氨基酸的平均加样回收率在88.25%~100.04%之间、RSD 为0.57%~2.73%(n=6), 水解氨基酸的平均加样回收率在90.21%~100.01%之间、RSD 为0.52%~2.32%(n=6)。结论 该方法稳定、快速、精密度高、线性范围宽, 适合杨树花中游离氨基酸和水解氨基酸的含量测定。

英文摘要:

      Objective To determine the contents of 21 free and hydrolyzed amino acids from Flos Populi Tomentosae by pre-column derivatization reversed phase high performance liquid chromatography (RP-HPLC). Methods The samples were derived with phenylisothiocyanate (PITC) and HPLC method was adopted. Gradient elution chromatography was applied to determine free and hydrolyzed amino acids con-tents in Flos Populi Tomentosae. Results The 21 amino acids showed a good linearity in the ranges of 0.0019?2.7 μmol?mL-1 with the correlation coefficients over 0.9990. The average recoveries of free amino acids were 88.25%?100.04% with relative standard deviation of 0.57%?2.73% (n=6); and the average reco-veries of total amino acids were 90.21%?100.01% with relative standard deviation of 0.52%?2.32%(n=6). Conclusion The method is sensitive, accurate and reproducible, and it is suitable for the determination of amino acids in many other samples.