磁固相萃取与试剂盒联用快速筛查食品中 苏丹红I的方法研究

Supported by National Natural Science Foundation of China (91217309, 31371843, 21005057) and the Fundamental Research Funds for the Central Universities (2042014kf0262)

中文关键词:  磁固相萃取  试剂盒  辣椒食品  苏丹红I

英文关键词:magnetic solid-phase extraction  kit  foodstuffs  Sudan I

中文摘要:

      目的 对试剂盒检测苏丹红I的方法进行改进, 提高试剂盒检测方法的灵敏度和准确性。方法 样品由磁性聚吡咯材料预处理后使用苏丹红I试剂盒进行检测。结果 结合磁固相萃取的试剂盒检测方法, 提高了对苏丹红I的检测灵敏度, 并将其用于食品中苏丹红I的检测, 方法检出限比直接用试剂盒检测降低了3倍。结论 由于磁固相萃取样品前处理方法简单、快速、溶剂用量少, 将其与试剂盒检测联用, 适于食品基质中苏丹红I的现场检测, 能有效避免试剂盒筛查方法的漏检情况, 减少假阴性结果的出现。

英文摘要:

      Objective To improve the detection sensitivity and accuracy of kits for real samples. Methods The Sudan I in food sample was extracted by magnetic polypyrrole sorbents before the detection by Sudan I kit. Results Compared to the direct detection by Sudan I kit, the sensitivity was improved when combining with magnetic solid-phase extraction (MSPE), and the detection limit of Sudan I in real food sample was reduced by 3 times. Conclusion As a simple, rapid and less solvent consuming method for sample pretreatment, the magnetic solid-phase extraction was suitable for rapid screening of Sudan I in foodstuffs in combination with the Sudan I kit. The proposed MSPE-Sudan I kit method greatly reduced false-negative results in screening Sudan I of real samples.