凝胶渗透色谱-气相色谱法测定猪肉中 11种有机磷农药残留

Determination of 11 organophosphorus pesticide multi-residues in pork by gas chromatography with gel permeation chromatography

中文关键词:  猪肉  凝胶色谱  气相色谱  有机磷  农药残留

英文关键词:pork  gel chromatography  gas chromatography  organophosphorus  pesticide residues

中文摘要:

      目的 建立凝胶色谱技术(GPC)净化, 气相色谱法测定猪肉中11种有机磷农药残留量的分析方法。方法 样品中的待测农药组分经丙酮提取, GPC净化去除油脂等杂质, 经旋蒸、氮吹、浓缩后, 进入GC分析, 外标法定量。结果 有机磷在0.02~1 μg/mL浓度范围内具有良好的线性响应, 相关系数在0.999以上, 加标量在0.01 mg/kg时, 加标回收率在83.4%~105.6%之间, 相对标准偏差在2.15%~6.52%之间, 检出低限为0.01 mg/kg。结论 该方法简便、快速、分离度好, 适于批量样品的测定。

英文摘要:

      Objective To develop a method for determination of 11 organophosphorus pesticide residues in pork by gas chromatography (GC) with gel permeation chromatography (GPC). Methods The samples of pork were extracted by acetone, cleaned up by GPC to remove grease and other impurities. After spin steaming, nitrogen blowing, enrichment, the analyte was determined by GC using the external standard method. Results The method showed a good linearity over the range of 0.02~1 μg/mL for 11 organophosphorus pesticide residues, with correlation coefficients above 0.999. The recoveries were 83.4%~105.6% at the spiked level of 0.01 mg/kg, with relative standard deviation between 2.15%~6.52%. The low detection limit was 0.01 mg/kg. Conclusion The method is simple, rapid, and has a good separating degree, and it is suitable for the batch sample determination.