马铃薯青枯病菌日常快速检验方法的对比

Comparison of routinequick tests for solanacearum in potato

中文关键词:  马铃薯  青枯病菌  疫情防控  PCR  双抗夹心酶联免疫吸附法

英文关键词:potatoes  solanacearum  epidemic prevention and control  PCR  double antibody sandwich enzyme linked immunosorbent assay

中文摘要:

      目的 对马铃薯青枯病菌日常快速检验的方法的对比。方法 以日常监管抽检的出口马铃薯为材料, 采用双抗夹心酶联免疫吸附法(double antibody sandwich enzyme linked immunosorbent assay, DAS-ELISA)以及分子生物学方法的实时荧光PCR(RT-PCR)法进行快速检测。结果 通过连续多批次马铃薯检测的结果进行统计分析, 试验结果显示, 应用上述两种方法对马铃薯青枯病菌携带情况具有较高的灵敏度, 特别是对处于潜伏期感染而没有表现出症状的马铃薯块茎的检测更为有效。结论 DAS-ELISA以及分子生物学方法的RT-PCR法对于马铃薯出口的快速检验快速通关具有重要的意义。

英文摘要:

      Objective To explore the epidemic prevention and control of solanacearum in potato. Methods With routine supervision sampling potato as the material, the serology agglutination double antibody sandwich enzyme linked immunosorbent assay (DAS ELISA) and molecular biology method of real-time fluorescent PCR (RT-PCR) method were adopted for quick detection. Results Continuous multiple statistical analysis of the testing results of the batch of potato test showed that the application of the above two methods of potato solanacearum carrying case had a high sensitivity, especially for in latent infection without showing symptoms of potato tuber detection showing more effective. Conlusion Both kinds of methods were of great importance for potato solanacearum epidemic prevention and export detection.