免疫亲和净化-光化学衍生-高效液相色谱法 检测动物肝脏中六种黄曲霉毒素

Determination of six aflatoxins in animal liver by photochemical derivative-high performance liquid chromatography combining with immunoaffinity column clean-up

中文关键词:  黄曲霉毒素  肝脏  高效液相色谱法  免疫亲和柱

英文关键词:aflatoxins  liver  high performance liquid chromatography  immunoaffinity column

中文摘要:

      目的 以鸡、猪等动物肝脏为研究对象, 二氯甲烷为提取溶剂, 建立免疫亲和柱净化、高效液相色谱-光化学衍生-荧光检测器检测六种黄曲霉毒素的分析方法。方法 样品经提取、过滤、浓缩、复溶后经免疫亲和柱净化, 进样后采用光化学衍生器在线对黄曲霉毒素G1、B1衍生, 荧光检测器分析B1、B2、G1、G2、M1、M2六种黄曲霉毒素。结果 在不同加标水平, 不同肝脏回收率在70.2%~94.1%之间, 变异系数为2.67%~12.90%, 检测低限为0.16 μg/kg。结论 该方法的准确度与精密度较高, 可以满足痕量分析要求。

英文摘要:

      Objective To develop a method for detecting six aflatoxins in chicken and pig liver. Methods Samples were extracted, filtered, concentrated and reconstituted purified by immunoaffinity column, derivatived aflatoxin G1, B1 using photochemical derivatization unit online after injection. Then six aflatoxins B1, B2, G1, G2, M1, and M2 were analyzed by fluorescence detector. Results The recoveries of samples with different spiking levels were 70.2%~94.1%. The RSD were 2.67%~12.90%. The lowest limit of quantification was 0.16 μg/kg. Conclusion This method is of high accuracy and precision, and can meet the requirements for trace analysis.