麻痹性贝类毒素受体蛋白Saxiphilin研究进展

Progress of paralytic shellfish toxin receptor proteins Saxiphilin

中文关键词:  Saxiphilin  麻痹性贝类毒素  石房蛤毒素  转铁蛋白

英文关键词:Saxiphilin  paralytic shellfish poison  saxitoxin  transferrin

中文摘要:

      麻痹性贝类毒素受体蛋白(Saxiphilin, SAX)是一种单体可溶性蛋白, 属于转铁蛋白家族, 具有转铁蛋白相似的组织分布但其不与Fe3 结合, 可能是一种转运和隔离内源性的有机分子的载体。SAX可高特异性地结合石房蛤毒素等多种麻痹性贝类毒素, 但不与竞争物河豚毒素结合, SAX广泛分布于牛蛙、节肢动物、鱼类、两栖类动物以及爬行动物中, 目前主要有两种方式获取SAX, 一是通过动物体内提取, 另一种是通过体内基因表达。本文从SAX蛋白的理化性质、结构和功能研究、分子进化等方面综述了该受体蛋白的研究进展, 并结合作者本身的研究工作, 展望了SAX受体蛋白建立多种麻痹性贝类毒素快速检测方法所存在的问题及应对策略, 揭示了SAX在贝类毒素检测及分析中具有广泛的应用前景。

英文摘要:

      Paralytic shellfish toxin (PSP) receptor protein (Saxiphilin, SAX) which belongs to the transferrin family, and has similar tissue distribution but not binding to Fe3 , is a soluble monomeric protein and a carrier of translocation of organic molecules and isolation of endogenous. SAX has high specificity combination with saxitoxin and other paralytic shellfish poisons with high affinity but not competitive tetrodotoxin, which widely distributed in arthropods, fish, amphibians and reptiles. There’re mainly two ways to obtain SAX, one is to extract by the animal, and the other is to gene express in vivo. In this paper, physical and chemical properties, structure and function, molecular evolution, applications and prospects of this receptor protein were reviewed. Combined with the author’s work, the existing problems of PSP detection and the corresponding strategies were put forward, which induced a wide range of application prospects in the detection and analysis of paralytic shellfish poisons.