桃花中总酚和总黄酮的提取及抗氧化活性研究

Extraction of total phenolics and flavanoids from peach blossoms and their antioxidant activity

中文关键词:  桃花  总酚  黄酮  提取  抗氧化

英文关键词:peach blossom  total phenolics  flavanoids  extraction  antioxidant

中文摘要:

      目的 研究提取条件对桃花总酚和总黄酮提取及提取物抗氧化活性的影响。方法 利用正交试验研究不同提取条件对桃花总酚和总黄酮提取的影响, 并进一步用Photochem抗氧化剂和自由基分析仪对各提取物的抗氧化活性进行分析测定。结果 桃花总酚和总黄酮提取的最佳工艺条件略有差异, 总酚提取条件为: 乙醇浓度50%(v/v)、提取时间2 h、温度50 ℃、提取次数3次; 总黄酮提取条件为: 乙醇浓度60%(v/v)、提取时间2 h、温度60 ℃、提取次数3次; 不同提取条件得到的桃花提取物对自由基都具有一定的清除作用, 但清除效果存在一定的差异。结论 提取条件不仅会影响桃花总酚和总黄酮的提取率, 而且可影响桃花提取物的抗氧化活性。

英文摘要:

      Objective This work aimed to evaluate the effects of extraction conditions on the yielding of total phenolics and flavanoids from peach blossoms as well as antioxidant activity of the extracts. Methods The optimal conditions for the extraction of total phenolics and flavanoids from peach blossoms were studied by orthogonal test, and the antioxidant activity of each extract was determined by using PHOTOCHEM? Pho-tochemiluminescence analyzer. Results The optimum condition for extraction of total phenolics from peach blossoms was at 50 ℃ of extraction temperature by using 50%(v/v) ethanol as solvent for 3 times, each time for 2 h. While for the extraction of total flavanoids, it was at 60 ℃ of extraction temperature by using 60%(v/v) ethanol as solvent for 3 times, each time for 2 h. Furthermore, the extracts obtained by different extraction con-ditions showed different antioxidant activities. Conclusion Extraction conditions could not only affect the yield of total phenolics and flavanoids, but also influence the antioxidant activity significantly.