| 李红权,孙良娟,吴晓薇,欧 安,刘中勇.环介导等温扩增技术检测食源性致病菌的研究进展[J].食品安全质量检测学报,2013,4(5):1445-1450 |
| 环介导等温扩增技术检测食源性致病菌的研究进展 |
| Research progress of LAMP technology in detection of foodborne pathogens |
| 投稿时间：2013-09-05 修订日期：2013-10-11 |
| DOI： |
| 中文关键词: 环介导等温扩增技术 食源性致病菌 检测应用 |
| 英文关键词:loop-mediated isothermal amplification foodborne pathogens application in detection |
| 基金项目:国家质量监督检验检疫总局科技项目(2013IK192)、湛江市财政资金科技专项(2012E0202) |
|
|
|
|
| 摘要点击次数: 1900 |
| 全文下载次数: 1560 |
| 中文摘要: |
| 环介导等温扩增(LAMP)技术是一种新型核酸扩增技术, 该技术在60?65 ℃恒温条件1 h内, 把特异性靶序列扩增到109水平, 具有高特异性、高敏感性、简单、便捷及成本低的特点, 已广泛应用于流行性细菌、病毒的定性定量检测等领域。本研究对LAMP的原理、特性及其应用做一综述, 从而为其在食源性致病菌检测应用中提供理论依据。 |
| 英文摘要: |
| Loop-mediated isothermal amplification (LAMP) technology is a new technology for nucleic acid amplification, which can amplify the specific target sequences to 109 level under the conditions of constant temperature at 60~65 ℃ within 1h. It has the characteristics of high specificity, sensitivity, simplicity, convenience and low cost. LAMP has been widely used in qualitative and quantitative detection of bacterial and viral epidemic.This article reviewed the principle, characteristics and application of LAMP technology, in order to provide theoret-ical basis for its application in the detection of foodborne pathogens. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
